Typical Testing Data/Standard Curve (for reference only)
Human Cell division cycle protein 20 homolog (CDC20) ELISA Kit | CDC20 elisa kit
Human Cell division cycle protein 20 homolog (CDC20) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: CDC20 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CDC20 antibody and an CDC20-HRP conjugate. The assay sample and buffer are incubated together with CDC20-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CDC20 concentration since CDC20 from samples and CDC20-HRP conjugate compete for the anti-CDC20 antibody binding site. Since the number of sites is limited, as more sites are occupied by CDC20 from the sample, fewer sites are left to bind CDC20-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CDC20 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
CDC20 appears to act as a regulatory protein interacting with several other proteins at multiple points in the cell cycle. It is required for two microtubule-dependent processes, nuclear movement prior to anaphase and chromosome separation. [provided by RefSeq, Jul 2008]
Uniprot Description
CDC20: Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1: in metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. CDC20-APC/C-induced degradation of NEUROD2 induces presynaptic differentiation. Found in a complex with CDC20, CDC27, SPATC1 and TUBG1. Interacts with SPATC1. Interacts with NEUROD2. Interacts with MAD2L1 and BUB1B. The phosphorylated form interacts with APC/C. Interacts with NINL. May interact with MAD2L2. Interacts with CDK5RAP2. Interacts with isoform 1 of NEK2. Belongs to the WD repeat CDC20/Fizzy family.
Protein type: Ubiquitin conjugating system; Cell cycle regulation
Chromosomal Location of Human Ortholog: 1p34.1
Cellular Component: nucleoplasm; spindle pole; centrosome; anaphase-promoting complex; perinuclear region of cytoplasm; cytoplasm; spindle; nucleus; cytosol
Molecular Function: protein C-terminus binding; protein binding; enzyme binding
Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; mitosis; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of meiosis; positive regulation of synaptic plasticity; regulation of dendrite development; protein ubiquitination; cell cycle; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex activation; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; cell division; mitotic cell cycle spindle assembly checkpoint; positive regulation of cell proliferation; mitotic cell cycle
Research Articles on CDC20
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Product Notes
The Human CDC20 cdc20 (Catalog #AAA7220893) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7220893 ELISA Kit recognizes Human CDC20. It is sometimes possible for the material contained within the vial of "Cell division cycle protein 20 homolog (CDC20), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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