Typical Testing Data/Standard Curve (for reference only)
(Human CD32/FCGR2b/c PicoKine ELISA Kit standard curve )
Human CD32/FCGR2b/c ELISA Kit | FCGR2B elisa kit
Human CD32/FCGR2b/c PicoKine ELISA Kit
Gene Names
FCGR2B; CD32; FCG2; CD32B; FCGR2; IGFR2
Reactivity
Human
Synonyms
CD32/FCGR2b/c; Human CD32/FCGR2b/c PicoKine ELISA Kit; Low affinity immunoglobulin gamma Fc region receptor II-b; Fc fragment of IgG; low affinity IIb; receptor (CD32); CD 32; CD32; CD32 antigen; CD32A; CD32B; CD32C; CDw32; Fc fragment of IgG low affinity II receptor for (CD32); Fc fragment of IgG low affinity IIa receptor; Fc fragment of IgG low affinity IIa receptor for (CD32); Fc fragment of IgG low affinity IIb receptor; Fc fragment of IgG low affinity IIb receptor for (CD32); Fc fragment of IgG low affinity IIc receptor; Fc fragment of IgG low affinity IIc receptor for (CD32); Fc gamma receptor IIB; Fc gamma RII a; Fc gamma RII b; Fc gamma RII c; Fc gamma RIIa; Fc gamma RIIb ; Fc gamma RIIc; Fc receptor; IgG; Fc-gamma RII-a; Fc-gamma RII-b; Fc-gamma RII-c; Fc-gamma-RIIa; FC-gamma-RIIB; Fc-GAMMA-RIIC; Fc[g]RII; FCG 2; FCG2; FCG2A_HUMAN; FcgammaRIIC; FCGR 2; FcGR; FCGR2; FCGR2A; FCGR2A1; FCGR2B; FCGR2C; Fcgr3; Fcgr3a; FcgRII; Fcr-2; Fcr-3; FcRII a; FCRII; FcRII b; FcRII c; FcRII-a; FcRIIC; IGFR 2; IGFR2; IgG Fc receptor II a; IgG Fc receptor II b; IgG Fc receptor II beta; IgG Fc receptor II c; IgG Fc receptor II-a; IgG Fc receptor II-b; IgG Fc receptor II-c; Immunoglobulin G Fc receptor II; Low affinity; immunoglobulin gamma Fc region receptor II a; Low affinity immunoglobulin gamma Fc region receptor II; Low affinity immunoglobulin gamma Fc region receptor II b; Low affinity immunoglobulin gamma Fc region receptor II c; Low affinity immunoglobulin gamma Fc region receptor II-a; Low affinity immunoglobulin gamma Fc region receptor II-c; Ly-17; Ly-m20; LyM-1; Lymphocyte antigen 17; MGC23887; MGC30032; FCGR2B elisa kit
Reactivity
Human
Specificity
Natural and recombinant human CD32
Sequence Length
310
Samples
Cell culture supernates, serum and plasma (heparin, EDTA)
Assay Type
Sandwich
Detection Range
46.9pg/ml-3000pg/ml
Sensitivity
< 1pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Typical Testing Data/Standard Curve (for reference only)
(Human CD32/FCGR2b/c PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only)
(Human CD32/FCGR2b/c PicoKine ELISA Kit standard curve )
Related Product Information for FCGR2B elisa kit
Principle of the assay: human CD32 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD32 has been precoated onto 96-well plates. Standards(NSO, A46-P217) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD32 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD32 amount of sample captured in plate.
Background: CD32 is a surface receptor protein and part of a large population of B cell co-receptors, which act to modulate signaling. It has a low-affinity for IgG antibodies and down-regulates antibody production in the presence of IgG. This feedback loops essentially lowers the production of IgG produced by B cells when there is a surplus in the body.
Background: CD32 is a surface receptor protein and part of a large population of B cell co-receptors, which act to modulate signaling. It has a low-affinity for IgG antibodies and down-regulates antibody production in the presence of IgG. This feedback loops essentially lowers the production of IgG produced by B cells when there is a surplus in the body.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
33,450 Da
NCBI Official Full Name
Low affinity immunoglobulin gamma Fc region receptor II-b
NCBI Official Synonym Full Names
Fc fragment of IgG, low affinity IIb, receptor (CD32)
NCBI Official Symbol
FCGR2B
NCBI Official Synonym Symbols
CD32; FCG2; CD32B; FCGR2; IGFR2
NCBI Protein Information
low affinity immunoglobulin gamma Fc region receptor II-b; CDw32; fcRII-b; Fc gamma RIIb; fc-gamma-RIIb; fc-gamma RII-b; igG Fc receptor II-b; Fc fragment of IgG, low affinity II, receptor for (CD32); Fc fragment of IgG, low affinity IIb, receptor for (CD32)
UniProt Protein Name
Low affinity immunoglobulin gamma Fc region receptor II-b
Protein Family
UniProt Gene Name
FCGR2B
UniProt Synonym Gene Names
CD32; FCG2; IGFR2; IgG Fc receptor II-b; Fc-gamma-RIIb; FcRII-b
UniProt Entry Name
FCG2B_HUMAN
NCBI Description
The protein encoded by this gene is a low affinity receptor for the Fc region of immunoglobulin gamma complexes. The encoded protein is involved in the phagocytosis of immune complexes and in the regulation of antibody production by B-cells. Variations in this gene may increase susceptibilty to systemic lupus erythematosus (SLE). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]
Uniprot Description
FCGR2B: a transmembrane receptor for the Fc region of complexed or aggregated immunoglobulin gamma (IgG). Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. Binding to this receptor results in down modulation of previous state of cell activation triggered via antigen receptors on B cells, T cells or via another Fc receptor. Three splice-variant isoforms have been observed.
Protein type: Cell surface; Membrane protein, integral; Oncoprotein
Chromosomal Location of Human Ortholog: 1q23
Cellular Component: plasma membrane; integral to membrane
Molecular Function: protein binding; IgG binding
Biological Process: regulation of immune response; viral reproduction; immune response; signal transduction
Disease: Systemic Lupus Erythematosus; Malaria, Susceptibility To
Research Articles on FCGR2B
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Product Notes
The Human FCGR2B fcgr2b (Catalog #AAA177376) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA177376 ELISA Kit recognizes Human FCGR2B. It is sometimes possible for the material contained within the vial of "CD32/FCGR2b/c, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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