Human CCL24/Eotaxin-2 ELISA Kit | CCL24 elisa kit

Human CCL24/Eotaxin-2 PicoKine ELISA Kit

Cat. Num. AAA176504

• Gene Names: CCL24; Ckb-6; MPIF2; MPIF-2; SCYA24
• Reactivity: Human
• Synonyms: CCL24/Eotaxin-2; Human CCL24/Eotaxin-2 PicoKine ELISA Kit; C-C motif chemokine 24; chemokine (C-C motif) ligand 24; C C motif chemokine 24; CCL24; Chemokine (C C motif) ligand 24; Chemokine CC Motif Ligand 2; CK beta 6; Ckb 6; Ckb6; Eosinophil chemotactic protein 2; Eotaxin 2 like protein; MPIF 2; MPIF2; Myeloid progenitor inhibitory factor 2; OTTHUMP00000210225; SCYA24; Small inducible cytokine A24; Small inducible cytokine subfamily A (Cys Cys) member 24; CCL24 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human CCL24
• Sequence Length: 119

• Samples: Cell culture supernates, serum and plasma (heparin, EDTA)
• Assay Type: Sandwich
• Detection Range: 78pg/ml-5000pg/ml
• Sensitivity: < 2pg/ml
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Human CCL24/Eotaxin-2 PicoKine ELISA Kit standard curve )

Related Product Information for CCL24 elisa kit

Principle of the assay: human CCL24 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CCL24 has been precoated onto 96-well plates. Standards(E.coli,V27-C119) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL24 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CCL24 amount of sample captured in plate.
Background: Chemokine (C-C motif) ligand 24 (CCL24) also known as myeloid progenitor inhibitory factor 2 (MPIF-2) or eosinophil chemotactic protein 2 (eotaxin-2) is a protein that in humans is encoded by the CCL24 gene.1 By use ofPCR analysis of somatic cell hybrid DNAs, radiation hybrid mapping, and a chromosome 7-specific YAC library, mapped the SCYA24 gene to chromosome 7q11.23.2 CCL24 is a small cytokine belonging to the CC chemokinefamily. CCL24 interacts with chemokine receptor CCR3 to induce chemotaxis in eosinophils.3 This chemokine isalso strongly chemotactic for resting T lymphocytes and slightly chemotactic for neutrophils.

NCBI and Uniprot Product Information

• NCBI GI #: 6920081
• NCBI GeneID: 6369
• UniProt Accession #: O00175; B2R5K2
• Molecular Weight: 13,134 Da
• NCBI Official Full Name: C-C motif chemokine 24
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 24
• NCBI Official Symbol: CCL24
• NCBI Official Synonym Symbols: Ckb-6; MPIF2; MPIF-2; SCYA24
• NCBI Protein Information: C-C motif chemokine 24; CK-beta-6; eotaxin-2; small-inducible cytokine A24; eosinophil chemotactic protein 2; myeloid progenitor inhibitory factor 2; small inducible cytokine subfamily A (Cys-Cys), member 24
• UniProt Protein Name: C-C motif chemokine 24
• Protein Family: C-C motif chemokine
• UniProt Gene Name: CCL24
• UniProt Synonym Gene Names: MPIF2; SCYA24; MPIF-2
• UniProt Entry Name: CCL24_HUMAN

NCBI Description

This gene belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity on resting T lymphocytes, a minimal activity on neutrophils, and is negative on monocytes and activated T lymphocytes. The protein is also a strong suppressor of colony formation by a multipotential hematopoietic progenitor cell line. [provided by RefSeq, Jul 2008]

Uniprot Description

CCL24: Chemotactic for resting T-lymphocytes, and eosinophils. Has lower chemotactic activity for neutrophils but none for monocytes and activated lymphocytes. Is a strong suppressor of colony formation by a multipotential hematopoietic progenitor cell line. Binds to CCR3. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 7q11.23

Cellular Component: extracellular space

Molecular Function: chemokine activity

Biological Process: cytoskeleton organization and biogenesis; signal transduction; chemotaxis; regulation of cell shape; positive regulation of angiogenesis; positive regulation of actin filament polymerization; cell-cell signaling; eosinophil chemotaxis; positive regulation of endothelial cell proliferation; immune response; inflammatory response; positive regulation of inflammatory response; positive regulation of cell migration