Human CCL18/PARC ELISA Kit | CCL18 elisa kit

Human CCL18/PARC PicoKine ELISA Kit

Cat. Num. AAA176563

• Gene Names: CCL18; CKb7; PARC; AMAC1; DCCK1; MIP-4; AMAC-1; DC-CK1; SCYA18
• Reactivity: Human
• Synonyms: CCL18/PARC; Human CCL18/PARC PicoKine ELISA Kit; C-C motif chemokine 18; chemokine (C-C motif) ligand 18 (pulmonary and activation-regulated); Alternative macrophage activation associated CC chemokine 1; Alternative macrophage activation-associated CC chemokine 1; AMAC 1; AMAC-1; AMAC1; C C motif chemokine 18; CC chemokine ligand 18; CC chemokine PARC; CCL18; CCL18(4-69); CCL18_HUMAN; Chemokine (C C motif) ligand 18; Chemokine (C C) dendritic; Chemokine ligand 18; CKb7; DC CK1; DC-CK1; DCCK1; Dendritic cell chemokine 1; Macrophage inflammatory protein 4; MIP 4; MIP-4; MIP4; PARC; Pulmonary and activation regulated chemokine; Pulmonary and activation-regulated chemokine; Small inducible cytokine A18; Small-inducible cytokine A18; CCL18 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human CCL18
• Sequence Length: 89

• Samples: Cell culture supernatants and serum
• Detection Range: 7.8pg/ml-500pg/ml
• Sensitivity: <10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CCL18 elisa kit

Principle of the Assay: The Picokine™ Human CCL18 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human CCL18 with a 96-well strip plate that is pre-coated with antibody specific for CCL18. The detection antibody is a biotinylated antibody specific for CCL18. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human CCL18 with immunogen: Expression system for standard: E.coli; Immunogen sequence: A21-A89. The kit is analytically validated with ready to use reagents. To measure Human CCL18, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human CCL18 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human CCL18 in the sample.

Background/Introduction: Chemokine(C-C motif) ligand 18(CCL18) is a small cytokine belonging to the CC chemokine family that was previously called PARC(pulmonary and activation-regulated chemokine). CCL18 is approximately 60% identical in amino acid sequence to CCL3. By analysis of a previously mapped CCL18 from 17q11.2, it is determined that the PARC gene is located within 1 of the 2 clusters of CC chemokine genes in this region. It is expressed at high levels in lung and at lower levels in certain lymphoid tissues, such as the lymph nodes, and is chemotactic for activated T cells and nonactivated lymphocytes. Beside, CCL18 recruits Th2 cells and basophils and may play a predominant role in allergic asthma.

NCBI and Uniprot Product Information

• NCBI GI #: 2493666
• NCBI GeneID: 6362
• UniProt Accession #: P55774; Q53X71; B5BUM2
• Molecular Weight: 9,849 Da
• NCBI Official Full Name: C-C motif chemokine 18
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 18 (pulmonary and activation-regulated)
• NCBI Official Symbol: CCL18
• NCBI Official Synonym Symbols: CKb7; PARC; AMAC1; DCCK1; MIP-4; AMAC-1; DC-CK1; SCYA18
• NCBI Protein Information: C-C motif chemokine 18; CC chemokine PARC; CC chemokine ligand 18; chemokine (C-C), dendritic; dendritic cell chemokine 1; small inducible cytokine A18; small-inducible cytokine A18; macrophage inflammatory protein 4; pulmonary and activation-regulated chemokine; alternative macrophage activation-associated CC chemokine 1; small inducible cytokine subfamily A (Cys-Cys), member 18, pulmonary and activation-regulated
• UniProt Protein Name: C-C motif chemokine 18
• Protein Family: C-C motif chemokine
• UniProt Gene Name: CCL18
• UniProt Synonym Gene Names: AMAC1; DCCK1; MIP4; PARC; SCYA18; AMAC-1; DC-CK1; MIP-4
• UniProt Entry Name: CCL18_HUMAN

NCBI Description

This antimicrobial gene is one of several Cys-Cys (CC) cytokine genes clustered on the q arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for naive T cells, CD4+ and CD8+ T cells and nonactivated lymphocytes, but not for monocytes or granulocytes. This chemokine attracts naive T lymphocytes toward dendritic cells and activated macrophages in lymph nodes. It may play a role in both humoral and cell-mediated immunity responses. [provided by RefSeq, Sep 2014]

Uniprot Description

CCL18: Chemotactic factor that attracts lymphocytes but not monocytes or granulocytes. May be involved in B-cell migration into B-cell follicles in lymph nodes. Attracts naive T-lymphocytes toward dendritic cells and activated macrophages in lymph nodes, has chemotactic activity for naive T-cells, CD4+ and CD8+ T-cells and thus may play a role in both humoral and cell-mediated immunity responses. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis; Chemokine

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: extracellular space

Molecular Function: protein binding; chemokine activity

Biological Process: cell-cell signaling; response to biotic stimulus; cell communication; immune response; signal transduction; inflammatory response; chemotaxis