Rabbit AMBRA1 Polyclonal Antibody | anti-AMBRA1 antibody

AMBRA1 Rabbit Polyclonal

Cat. Num. AAA766588

• Gene Names: AMBRA1; DCAF3; WDR94
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Immunohistochemistry, Western Blot, Immunofluorescence
• Purity: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Synonyms: AMBRA1; Polyclonal Antibody; AMBRA1 Rabbit Polyclonal; anti-AMBRA1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 1238

• Applicable Applications for anti-AMBRA1 antibody: ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB), Immunofluorescence (IF)
• Immunogen: Autophagy/beclin-1 regulator 1
• Preparation and Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded rat kidney using MBS766588 (AMBRA1 antibody) at dilution of 1:50)

Western Blot (WB)

(mouse brain tissue were subjected to SDS PAGE followed by western blot with MBS766588 (AMBRA1 antibody) at dilution of 1:1000)

NCBI and Uniprot Product Information

• NCBI GI #: 663429567
• NCBI GeneID: 55626
• NCBI Accession #: NP_001287660.1
• NCBI GenBank Nucleotide #: NM_001300731.1
• Molecular Weight: 84,428 Da
• NCBI Official Full Name: activating molecule in BECN1-regulated autophagy protein 1 isoform 4
• NCBI Official Synonym Full Names: autophagy and beclin 1 regulator 1
• NCBI Official Symbol: AMBRA1
• NCBI Official Synonym Symbols: DCAF3; WDR94
• NCBI Protein Information: activating molecule in BECN1-regulated autophagy protein 1
• UniProt Protein Name: Activating molecule in BECN1-regulated autophagy protein 1
• Protein Family: Activating molecule in BECN1-regulated autophagy protein
• UniProt Gene Name: AMBRA1
• UniProt Synonym Gene Names: KIAA1736
• UniProt Entry Name: AMRA1_HUMAN

Uniprot Description

AMBRA1: a WD40 protein that regulates autophagy and development of the nervous system. A highly conserved vertebrate protein whose expression is mostly confined to the neuroepithelium during early neurogenesis. Involved in autophagy in controlling protein turnover during neuronal development, and in regulating normal cell survival and proliferation. Interacts with ATG6. Forms a complex with ATG6 and PIK3C3. Its functional inactivation in mouse is lethal in utero (starting from embryonic day 14.5), leading to severe neural tube defects associated with autophagy impairment, accumulation of ubiquitinated proteins, and excessive apoptosis. Hyperproliferation followed by a wave of caspase-dependent cell death was the earliest detectable abnormality in the developing neuroepithelium. Five isoforms of the human protein are produced by alternative splicing.

Protein type: Vesicle; Autophagy

Chromosomal Location of Human Ortholog: 11p11.2

Cellular Component: autophagic vacuole; axoneme; cytoplasm; cytosol; mitochondrial outer membrane; mitochondrion; perinuclear region of cytoplasm; phagocytic vesicle

Molecular Function: protein binding; ubiquitin protein ligase binding

Biological Process: cell differentiation; macroautophagy; negative regulation of cell proliferation; negative regulation of neuron apoptosis; neural tube development; positive regulation of phosphoinositide 3-kinase activity