Human Catenin Alpha 1 (CTNNa1) ELISA Kit | CTNNa1 elisa kit

Human Catenin Alpha 1 (CTNNa1) ELISA Kit

Cat. Num. AAA2020102

• Gene Names: CTNNA1; CAP102
• Reactivity: Human
• Synonyms: Catenin Alpha 1 (CTNNa1); Human Catenin Alpha 1 (CTNNa1) ELISA Kit; CTNN-A1; aCat; CAP102; Cadherin Associated Protein Alpha 1; Alpha E-catenin; Renal carcinoma antigen NY-REN-13; CTNNa1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Catenin Alpha 1 (CTNNa1).; No significant cross-reactivity or interference between Catenin Alpha 1 (CTNNa1) and analogues was observed.
• Sequence Length: 906

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.054ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Catenin Alpha 1 (CTNNa1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Catenin Alpha 1 (CTNNa1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CTNNa1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Catenin Alpha 1 (CTNNa1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Catenin Alpha 1 (CTNNa1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Catenin Alpha 1 (CTNNa1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Catenin Alpha 1 (CTNNa1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for CTNNa1 elisa kit

Developmental science

NCBI and Uniprot Product Information

• NCBI GI #: 55770844
• NCBI GeneID: 1495
• NCBI Accession #: NP_001894.2
• NCBI GenBank Nucleotide #: NM_001903.3
• UniProt Accession #: P35221; Q12795; Q8N1C0
• Molecular Weight: 59,550 Da
• NCBI Official Full Name: catenin alpha-1 isoform 1
• NCBI Official Synonym Full Names: catenin (cadherin-associated protein), alpha 1, 102kDa
• NCBI Official Symbol: CTNNA1
• NCBI Official Synonym Symbols: CAP102
• NCBI Protein Information: catenin alpha-1
• UniProt Protein Name: Catenin alpha-1
• Protein Family: Catenin
• UniProt Gene Name: CTNNA1
• UniProt Entry Name: CTNA1_HUMAN

Uniprot Description

CTNNA1: Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherens junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. May play a crucial role in cell differentiation. Monomer and homodimer; the monomer preferentially binds to CTNNB1 and the homodimer to actin. Binds MLLT4 and F-actin. Possible component of an E-cadherin/ catenin adhesion complex together with E-cadherin/CDH1 and beta-catenin/CTNNB1 or gamma- catenin/JUP; the complex is located to adherens junctions. The stable association of CTNNA1 is controversial as CTNNA1 was shown not to bind to F-actin when assembled in the complex. Alternatively, the CTNNA1-containing complex may be linked to F- actin by other proteins such as LIMA1. Interacts with ARHGAP21 and with AJUBA. Interacts with LIMA1. Expressed ubiquitously in normal tissues. Belongs to the vinculin/alpha-catenin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Actin-binding

Chromosomal Location of Human Ortholog: 5q31.2

Cellular Component: focal adhesion; lamellipodium; acrosome; plasma membrane; intercellular junction; catenin complex; zonula adherens; cytosol; actin cytoskeleton

Molecular Function: actin filament binding; protein binding; cadherin binding; gamma-catenin binding; beta-catenin binding; structural molecule activity; vinculin binding

Biological Process: intercellular junction assembly and maintenance; apical junction assembly; gap junction assembly; protein heterooligomerization; axon regeneration; male gonad development; establishment and/or maintenance of cell polarity; actin filament organization; odontogenesis of dentine-containing teeth; muscle cell differentiation; ovarian follicle development; negative regulation of neuroblast proliferation; response to estrogen stimulus; positive regulation of muscle cell differentiation; positive regulation of smoothened signaling pathway; cell adhesion; vascular endothelial growth factor receptor signaling pathway; aging