Human CASP2 (Caspase-2) ELISA Kit | CASP2 elisa kit

Human CASP2 (Caspase-2) ELISA Kit

Cat. Num. AAA7606693

• Gene Names: CASP3; CPP32; SCA-1; CPP32B
• Reactivity: Human
• Synonyms: CASP2 (Caspase-2); Human CASP2 (Caspase-2) ELISA Kit; ICH1; NEDD2; Neural precursor cell expressed developmentally down-regulated protein 2; NEDD-2; Protease ICH-1; CASP2 elisa kit

• Reactivity: Human
• Sequence Length: 277

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 78.125-5000pg/ml
• Sensitivity: 46.875pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CASP2 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- CASP2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-CASP2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the CASP2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of CASP2 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 77416852
• NCBI GeneID: 836
• UniProt Accession #: P42574; Q96AN1; Q96KP2; A8K5M2; D3DP53
• Molecular Weight: 31,608 Da
• NCBI Official Full Name: Caspase-3
• NCBI Official Synonym Full Names: caspase 3
• NCBI Official Symbol: CASP3
• NCBI Official Synonym Symbols: CPP32; SCA-1; CPP32B
• NCBI Protein Information: caspase-3
• UniProt Protein Name: Caspase-3
• Protein Family: Caspase
• UniProt Gene Name: CASP3
• UniProt Synonym Gene Names: CPP32; CASP-3; CPP-32; SCA-1

NCBI Description

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq, Jul 2008]

Uniprot Description

CASP3: Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop- helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit. Interacts with BIRC6/bruce. Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system. Inhibited by isatin sulfonamides. Belongs to the peptidase C14A family.

Protein type: Apoptosis; EC 3.4.22.56; Motility/polarity/chemotaxis; Protease

Chromosomal Location of Human Ortholog: 4q35.1

Cellular Component: cytosol; nucleoplasm; nucleus; plasma membrane

Molecular Function: aspartic-type endopeptidase activity; caspase activator activity; cysteine-type endopeptidase activity; peptidase activity; protein binding

Biological Process: apoptosis; caspase activation; caspase activation via cytochrome c; DNA fragmentation during apoptosis; erythrocyte differentiation; keratinocyte differentiation; negative regulation of apoptosis; nerve growth factor receptor signaling pathway; neuron differentiation; platelet formation; proteolysis; regulation of macroautophagy