Human Cartilage Associated Protein (CRTAP) ELISA Kit | CRTAP elisa kit

Human Cartilage Associated Protein (CRTAP) ELISA Kit

Cat. Num. AAA2706671

• Gene Names: CRTAP; OI7; CASP; P3H5; LEPREL3
• Reactivity: Human
• Synonyms: Cartilage Associated Protein (CRTAP); Human Cartilage Associated Protein (CRTAP) ELISA Kit; CASP; LEPREL3; Leprecan-Like 3; CRTAP elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cartilage Associated Protein (CRTAP).; No significant cross-reactivity or interference between Cartilage Associated Protein (CRTAP) and analogues was observed.
• Sequence Length: 401

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.061ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cartilage Associated Protein (CRTAP) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cartilage Associated Protein (CRTAP) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CRTAP elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cartilage Associated Protein (CRTAP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cartilage Associated Protein (CRTAP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cartilage Associated Protein (CRTAP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cartilage Associated Protein (CRTAP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 5453601
• NCBI GeneID: 10491
• NCBI Accession #: NP_006362.1
• NCBI GenBank Nucleotide #: NM_006371.4
• UniProt Accession #: O75718; B2RBL6
• Molecular Weight: 46,562 Da
• NCBI Official Full Name: cartilage-associated protein
• NCBI Official Synonym Full Names: cartilage associated protein
• NCBI Official Symbol: CRTAP
• NCBI Official Synonym Symbols: OI7; CASP; P3H5; LEPREL3
• NCBI Protein Information: cartilage-associated protein
• UniProt Protein Name: Cartilage-associated protein
• Protein Family: Cartilage-associated protein
• UniProt Gene Name: CRTAP
• UniProt Synonym Gene Names: CASP

NCBI Description

The protein encoded by this gene is similar to the chicken and mouse CRTAP genes. The encoded protein is a scaffolding protein that may influence the activity of at least one member of the cytohesin/ARNO family in response to specific cellular stimuli. Defects in this gene are associated with osteogenesis imperfecta, a connective tissue disorder characterized by bone fragility and low bone mass. [provided by RefSeq, Jul 2008]

Uniprot Description

Necessary for efficient 3-hydroxylation of fibrillar collagen prolyl residues.