Rabbit NRF1 Polyclonal Antibody | anti-NRF1 antibody

Anti-NRF1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8101872

• Gene Names: NRF1; ALPHA-PAL
• Reactivity: Human; Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
• Applications: Immunohistochemistry, Immunocytochemistry, Immunofluorescence
• Purity: Protein A & Antigen Affinity
• Synonyms: NRF1; Polyclonal Antibody; Anti-NRF1 Antibody; Rabbit Polyclonal; NRF1 Antibody; Rabbit PAb; Antigen Affinity Purified; nuclear respiratory factor 1; Anti-ALPHA-PAL Antibody; anti-NRF1 antibody

• Host: Rabbit
• Reactivity: Human; Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human NRF1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-NRF1 antibody: Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: IHC-P: 1:200-1:500; ICC/IF: 1:300-1:10,000
• Immunogen: A synthetic peptide corresponding to the N-terminus of the Human NRF1
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human NRF1, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human NRF1 in human skeletal muscle with rabbit polyclonal antibody (1:300, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC)

(Immunochemical staining of human NRF1 in human gastric cancer with rabbit polyclonal antibody (1:300, formalin-fixed paraffin embedded sections).)

Immunofluorescence (IF)

(Immunofluorescence staining of NRF1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human NRF1 polyclonal antibody (dilution ratio 1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to nucleus.)

Related Product Information for anti-NRF1 antibody

NRF1 (Nuclear Respiratory Factor 1) is a Protein Coding gene. This gene encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes. The encoded protein belongs to the NRF1/Ewg family. NRF1 serves as a transcription factor that activates the expression of a wide range of nuclear genes essential for mitochondrial biogenesis and function, including mitochondrial respiratory complex subunits, heme biosynthetic enzymes, and regulatory factors involved in the replication and transcription of mitochondrial DNA. NRF1 plays a crucial role in regulating the expression of a broad range of mitochondrial genes in the nucleus in response to cellular oxidative stress. The protein has also been associated with the regulation of neurite outgrowth.

NCBI and Uniprot Product Information

• NCBI GI #: 93141039
• NCBI GeneID: 4899
• NCBI Accession #: NP_001035199.1
• NCBI GenBank Nucleotide #: NM_001040110.1
• UniProt Accession #: Q16656; Q15305
• Molecular Weight: 53,541 Da
• NCBI Official Full Name: nuclear respiratory factor 1
• NCBI Official Synonym Full Names: nuclear respiratory factor 1
• NCBI Official Symbol: NRF1
• NCBI Official Synonym Symbols: ALPHA-PAL
• NCBI Protein Information: nuclear respiratory factor 1; NRF-1; alpha palindromic-binding protein
• UniProt Protein Name: Nuclear respiratory factor 1
• Protein Family: Nuclear respiratory factor
• UniProt Gene Name: NRF1
• UniProt Synonym Gene Names: NRF-1; Alpha-pal
• UniProt Entry Name: NRF1_HUMAN

NCBI Description

This gene encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. Alternate transcriptional splice variants, which encode the same protein, have been characterized. Additional variants encoding different protein isoforms have been described but they have not been fully characterized. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for "nuclear factor (erythroid-derived 2)-like 1" which has an official symbol of NFE2L1. [provided by RefSeq, Jul 2008]

Uniprot Description

NRF1: Transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. Links the transcriptional modulation of key metabolic genes to cellular growth and development. Implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. Belongs to the NRF1/Ewg family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 7q32

Cellular Component: nucleoplasm; cytoplasm

Biological Process: regulation of transcription from RNA polymerase II promoter; mitochondrion organization and biogenesis; organ regeneration; generation of precursor metabolites and energy; transcription, DNA-dependent; response to folic acid; organelle organization and biogenesis; response to hypoxia; positive regulation of transcription from RNA polymerase II promoter; response to lipopolysaccharide; cellular lipid metabolic process; response to electrical stimulus; response to estradiol stimulus