Human Carboxypeptidase N catalytic chain (CPN1) ELISA Kit | CPN1 elisa kit

Human Carboxypeptidase N catalytic chain (CPN1) ELISA Kit

Cat. Num. AAA7206235

• Gene Names: CPN1; CPN; SCPN
• Reactivity: Human
• Synonyms: Carboxypeptidase N catalytic chain (CPN1); Human Carboxypeptidase N catalytic chain (CPN1) ELISA Kit; CPN1 elisa kit

• Reactivity: Human

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve

Related Product Information for CPN1 elisa kit

Intended Uses: This CPN1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CPN1. This ELISA kit for research use only!

Principle of the Assay||CPN1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-CPN1 antibody and an CPN1-HRP conjugate. The assay sample and buffer are incubated together with CPN1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CPN1 concentration since CPN1 from samples and CPN1-HRP conjugate compete for the anti-CPN1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CPN1 from the sample, fewer sites are left to bind CPN1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CPN1 concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4503011
• NCBI GeneID: 1369
• NCBI Accession #: NP_001299.1
• NCBI GenBank Nucleotide #: NM_001308.2
• Molecular Weight: 52,286 Da
• NCBI Official Full Name: carboxypeptidase N catalytic chain
• NCBI Official Synonym Full Names: carboxypeptidase N, polypeptide 1
• NCBI Official Symbol: CPN1
• NCBI Official Synonym Symbols: CPN; SCPN
• NCBI Protein Information: carboxypeptidase N catalytic chain; kininase I; kininase-1; carboxypeptidase K; lysine carboxypeptidase; serum carboxypeptidase N; anaphylatoxin inactivator; arginine carboxypeptidase; plasma carboxypeptidase B; carboxypeptidase N small subunit; carboxypeptidase N catalytic subunit; carboxypeptidase N polypeptide 1 50 kD
• UniProt Protein Name: Carboxypeptidase N catalytic chain
• Protein Family: Carboxypeptidase
• UniProt Gene Name: CPN1
• UniProt Synonym Gene Names: ACBP; CPN; SCPN
• UniProt Entry Name: CBPN_HUMAN

NCBI Description

Carboxypeptidase N is a plasma metallo-protease that cleaves basic amino acids from the C terminal of peptides and proteins. The enzyme is important in the regulation of peptides like kinins and anaphylatoxins, and has also been known as kininase-1 and anaphylatoxin inactivator. This enzyme is a tetramer comprised of two identical regulatory subunits and two identical catalytic subunits; this gene encodes the catalytic subunit. Mutations in this gene can be associated with angioedema or chronic urticaria resulting from carboxypeptidase N deficiency. [provided by RefSeq, Jul 2008]

Uniprot Description

CPN1: Protects the body from potent vasoactive and inflammatory peptides containing C-terminal Arg or Lys (such as kinins or anaphylatoxins) which are released into the circulation. Defects in CPN1 are the cause of carboxypeptidase N deficiency (CPND). Patients affected present some combination of angioedema or chronic urticaria, as well as hay fever or astma, and have also slightly depressed serum carboxy peptidase N, suggestive of autosomal recessive inheritance of this disorder. Belongs to the peptidase M14 family.

Protein type: EC 3.4.17.3; Secreted; Secreted, signal peptide; Protease

Chromosomal Location of Human Ortholog: 10q24.2

Cellular Component: extracellular space

Molecular Function: zinc ion binding; metallocarboxypeptidase activity

Biological Process: response to glucocorticoid stimulus; proteolysis

Disease: Carboxypeptidase N Deficiency