Human carboxypeptidase N, polypeptide 1 ELISA Kit | CPN1 elisa kit

Human Carboxypeptidase N Catalytic chain, CPN1 ELISA Kit

Cat. Num. AAA946937

• Gene Names: CPN1; CPN; SCPN
• Reactivity: Human
• Synonyms: carboxypeptidase N; polypeptide 1; Human Carboxypeptidase N Catalytic chain; CPN1 ELISA Kit; Human Carboxypeptidase N catalytic chain (CPN1) ELISA kit; CPN; FLJ40792; SCPN; anaphylatoxin inactivator; arginine carboxypeptidase; carboxypeptidase N catalytic subunit; carboxypeptidase N polypeptide 1 50 kD; carboxypeptidase N small subunit; kininase I; lysin; CPN1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human CPN1. No significant cross-reactivity or interference between human CPN1 and analogues was observed.
• Sequence Length: 458

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 31.25 mU/ml-2000 mU/ml
• Sensitivity: < 7.8 mU/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CPN1 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CPN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CPN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CPN1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CPN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 4503011
• NCBI GeneID: 1369
• NCBI Accession #: NP_001299.1
• NCBI GenBank Nucleotide #: NM_001308.2
• UniProt Accession #: P15169; B1AP59
• Molecular Weight: 52,286 Da
• NCBI Official Full Name: carboxypeptidase N catalytic chain
• NCBI Official Synonym Full Names: carboxypeptidase N, polypeptide 1
• NCBI Official Symbol: CPN1
• NCBI Official Synonym Symbols: CPN; SCPN
• NCBI Protein Information: carboxypeptidase N catalytic chain; kininase I; kininase-1; carboxypeptidase K; lysine carboxypeptidase; serum carboxypeptidase N; anaphylatoxin inactivator; arginine carboxypeptidase; plasma carboxypeptidase B; carboxypeptidase N small subunit; carboxypeptidase N catalytic subunit; carboxypeptidase N polypeptide 1 50 kD
• UniProt Protein Name: Carboxypeptidase N catalytic chain
• Protein Family: Carboxypeptidase
• UniProt Gene Name: CPN1
• UniProt Synonym Gene Names: ACBP; CPN; SCPN
• UniProt Entry Name: CBPN_HUMAN

NCBI Description

Carboxypeptidase N is a plasma metallo-protease that cleaves basic amino acids from the C terminal of peptides and proteins. The enzyme is important in the regulation of peptides like kinins and anaphylatoxins, and has also been known as kininase-1 and anaphylatoxin inactivator. This enzyme is a tetramer comprised of two identical regulatory subunits and two identical catalytic subunits; this gene encodes the catalytic subunit. Mutations in this gene can be associated with angioedema or chronic urticaria resulting from carboxypeptidase N deficiency. [provided by RefSeq, Jul 2008]

Uniprot Description

CPN1: Protects the body from potent vasoactive and inflammatory peptides containing C-terminal Arg or Lys (such as kinins or anaphylatoxins) which are released into the circulation. Defects in CPN1 are the cause of carboxypeptidase N deficiency (CPND). Patients affected present some combination of angioedema or chronic urticaria, as well as hay fever or astma, and have also slightly depressed serum carboxy peptidase N, suggestive of autosomal recessive inheritance of this disorder. Belongs to the peptidase M14 family.

Protein type: EC 3.4.17.3; Secreted; Protease; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 10q24.2

Cellular Component: extracellular space

Molecular Function: zinc ion binding; metallocarboxypeptidase activity

Biological Process: response to glucocorticoid stimulus; proteolysis

Disease: Carboxypeptidase N Deficiency