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Typical Testing Data/Standard Curve (for reference only)

Human Carboxylesterase 2 (CES2) ELISA Kit | CES2 elisa kit

Human Carboxylesterase 2 (CES2) Wide-range ELISA Kit

Gene Names
CES2; iCE; CE-2; PCE-2; CES2A1
Reactivity
Human
Synonyms
Carboxylesterase 2 (CES2); Human Carboxylesterase 2 (CES2) Wide-range ELISA Kit; CE-2; CES2A1; PCE-2; iCE; Intestine; Liver; Methylumbelliferyl-acetate deacetylase 2; Cocaine esterase; CES2 elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of CES2. No significant cross-reactivity or interference between CES2 and analogues was observed.
Sequence Length
623
Samples
Human serum, plasma, tissue homogenates and other biological fluids
Detection Range
0.625-40ng/mL
Sensitivity
< 0.245ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CES2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CES2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CES2 elisa kit
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to CES2. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to CES2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CES2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CES2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for CES2 elisa kit

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
59,959 Da
NCBI Official Full Name
cocaine esterase isoform 1
NCBI Official Synonym Full Names
carboxylesterase 2
NCBI Official Symbol
CES2
NCBI Official Synonym Symbols
iCE; CE-2; PCE-2; CES2A1
NCBI Protein Information
cocaine esterase
UniProt Protein Name
Cocaine esterase
Protein Family
UniProt Gene Name
CES2
UniProt Synonym Gene Names
ICE; CE-2; hCE-2

NCBI Description

This gene encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. The protein encoded by this gene is the major intestinal enzyme and functions in intestine drug clearance. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Oct 2010]

Uniprot Description

CES2: Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Shows high catalytic efficiency for hydrolysis of cocaine, 4-methylumbelliferyl acetate, heroin and 6-monoacetylmorphine. Belongs to the type-B carboxylesterase/lipase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.1.1; EC 3.1.1.56; EC 3.1.1.84; Endoplasmic reticulum; Hydrolase; Xenobiotic Metabolism - drug metabolism - other enzymes

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: endoplasmic reticulum; endoplasmic reticulum lumen; extracellular space

Biological Process: catabolic process; xenobiotic metabolic process

Research Articles on CES2

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Product Notes

The Human CES2 ces2 (Catalog #AAA2088282) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2088282 ELISA Kit recognizes Human CES2. It is sometimes possible for the material contained within the vial of "Carboxylesterase 2 (CES2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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