Typical Testing Data/Standard Curve (for reference only)
Human cAMP responsive element modulator (CREM) ELISA Kit | CREM elisa kit
Human cAMP responsive element modulator (CREM) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: Cre ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-Cre antibody and an Cre-HRP conjugate. The assay sample and buffer are incubated together with Cre-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Cre concentration since Cre from samples and Cre-HRP conjugate compete for the anti-Cre antibody binding site. Since the number of sites is limited, as more sites are occupied by Cre from the sample, fewer sites are left to bind Cre-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Cre concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a bZIP transcription factor that binds to the cAMP responsive element found in many viral and cellular promoters. It is an important component of cAMP-mediated signal transduction during the spermatogenetic cycle, as well as other complex processes. Alternative promoter and translation initiation site usage allows this gene to exert spatial and temporal specificity to cAMP responsiveness. Multiple alternatively spliced transcript variants encoding several different isoforms have been found for this gene, with some of them functioning as activators and some as repressors of transcription. [provided by RefSeq, Jul 2008]
Uniprot Description
CREM: a bZIP transcription factor that binds to the cAMP responsive element found in many viral and cellular promoters. An important component of cAMP-mediated signal transduction during the spermatogenetic cycle, as well as other complex processes. Alternative splice isoforms allow this protein to exert spatial and temporal specificity to cAMP responsiveness. Twentyeight alternatively spliced isoforms of the human protein have been described, with some of them functioning as activators and some as repressors of transcription.
Protein type: DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 10p11.21
Cellular Component: transcription factor complex; cytoplasm; nucleus
Molecular Function: protein binding; DNA binding; cAMP response element binding protein binding; transcription factor activity
Biological Process: response to cAMP; transcription, DNA-dependent; regulation of transcription, DNA-dependent; multicellular organismal development; spermatogenesis; positive regulation of transcription from RNA polymerase II promoter; glycosphingolipid metabolic process; circadian regulation of gene expression; cell differentiation; negative regulation of transcription, DNA-dependent; signal transduction
Research Articles on CREM
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Product Notes
The Human CREM crem (Catalog #AAA7238165) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7238165 ELISA Kit recognizes Human CREM. It is sometimes possible for the material contained within the vial of "cAMP responsive element modulator (CREM), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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