Human Calmegin (CLGN) ELISA Kit | CLGN elisa kit
Human Calmegin (CLGN)(Sandwich ELISA) ELISA Kit
Reactivity
Human
Applications
ELISA
Synonyms
Calmegin (CLGN); Human Calmegin (CLGN)(Sandwich ELISA) ELISA Kit; ELISA Kit for Calmegin (CLGN); CLGN/Calmegin; CLGN elisa kit
Reactivity
Human
Specificity
Reactive with Human Calmegin (CLGN)
This assay has high sensitivity and excellent specificity for detection of Calmegin (CLGN). No significant cross-reactivity or interference between Calmegin (CLGN) and analogues was observed.
This assay has high sensitivity and excellent specificity for detection of Calmegin (CLGN). No significant cross-reactivity or interference between Calmegin (CLGN) and analogues was observed.
Applicable Applications for CLGN elisa kit
ELISA (EIA)
Application Notes
Enzyme-linked immunosorbent assay for Antigen Detection.
Assay Type
Sandwich ELISA
Sample Types
tissue homogenates, cell lysates and other biological fluids
Detection Method
Double-antibody Sandwich
Detection Range
0.312-20ng/mL
Sensitivity
0.118ng/mL
Assay Length
3 h
Sample Size
100ul (Please refer to the manual for the specific data.)
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Calmegin (CLGN) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calmegin (CLGN) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calmegin (CLGN) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Assay Procedure Summary
1. Prepare all reagents, samples and standards
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37 degree C
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37 degree C
4. Aspirate and wash 3 times
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37 degree C
6. Aspirate and wash 5 times
7. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37 degree C
8. Add 50ul Stop Solution. Read at 450nm immediately.
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37 degree C
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37 degree C
4. Aspirate and wash 3 times
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37 degree C
6. Aspirate and wash 5 times
7. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37 degree C
8. Add 50ul Stop Solution. Read at 450nm immediately.
Test Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Histone H4 (H4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Histone H4 (H4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Histone H4 (H4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Histone H4 (H4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Preparation and Storage
Store at 4 degree C for 1 month/ -20 degree C for long-term (within shelf life).
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Product Categories/Family for CLGN elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
70
NCBI Official Full Name
calmegin
NCBI Official Synonym Full Names
calmegin
NCBI Official Symbol
CLGN
NCBI Protein Information
calmegin
UniProt Protein Name
Calmegin
Protein Family
UniProt Gene Name
CLGN
NCBI Description
Calmegin is a testis-specific endoplasmic reticulum chaperone protein. CLGN may play a role in spermatogeneisis and infertility. [provided by RefSeq, Jul 2008]
Uniprot Description
CLGN: Probably plays an important role in spermatogenesis. Binds calcium ions. Belongs to the calreticulin family.
Protein type: Membrane protein, integral; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 4q31.1
Cellular Component: endoplasmic reticulum
Biological Process: single fertilization
