Human Calcium/calmodulin dependent protein kinase type IV (CAMK4) ELISA Kit | CAMK4 elisa kit

Human Calcium/calmodulin dependent protein kinase type IV (CAMK4) ELISA Kit

Cat. Num. AAA7230332

• Gene Names: CAMK4; IV; caMK; CaMK IV; CaMK-GR
• Reactivity: Human
• Synonyms: Calcium/calmodulin dependent protein kinase type IV (CAMK4); Human Calcium/calmodulin dependent protein kinase type IV (CAMK4) ELISA Kit; CAMK4 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of CAMK4. No significant cross-reactivity or interference between CAMK4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CAMK4 and all the analogues, therefore, cross reaction may still exist in some cases.

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 0.1 ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for CAMK4 elisa kit

Intended Uses: This CAMK4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CAMK4. This ELISA kit for research use only, not for therapeutic or test applications!

Principle of the Assay: CAMK4 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-CAMK4 antibody and a CAMK4-HRP conjugate. The assay sample and buffer are incubated together with CAMK4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CAMK4 concentration since CAMK4 from samples and CAMK4-HRP conjugate compete for the anti-CAMK4 antibody binding site. Since the number of sites is limited, as more sites are occupied by CAMK4 from the sample, fewer sites are left to bind CAMK4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CAMK4 concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4502557
• NCBI GeneID: 814
• NCBI Accession #: NP_001735.1
• NCBI GenBank Nucleotide #: NM_001744.4
• Molecular Weight: 51,926 Da
• NCBI Official Full Name: calcium/calmodulin-dependent protein kinase type IV
• NCBI Official Synonym Full Names: calcium/calmodulin-dependent protein kinase IV
• NCBI Official Symbol: CAMK4
• NCBI Official Synonym Symbols: IV; caMK; CaMK IV; CaMK-GR
• NCBI Protein Information: calcium/calmodulin-dependent protein kinase type IV; CAM kinase IV; caM kinase-GR; CAM kinase- GR; brain Ca++-calmodulin-dependent protein kinase type IV; brain Ca(2+)-calmodulin-dependent protein kinase type IV; calcium/calmodulin-dependent protein kinase type IV catalytic chain
• UniProt Protein Name: Calcium/calmodulin-dependent protein kinase type IV
• Protein Family: Calcium/calmodulin-dependent protein kinase
• UniProt Gene Name: CAMK4
• UniProt Synonym Gene Names: CAMK; CAMK-GR; CAMKIV; CaMK IV
• UniProt Entry Name: KCC4_HUMAN

NCBI Description

The product of this gene belongs to the serine/threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. This enzyme is a multifunctional serine/threonine protein kinase with limited tissue distribution, that has been implicated in transcriptional regulation in lymphocytes, neurons and male germ cells. [provided by RefSeq, Jul 2008]

Uniprot Description

CAMK4: a protein kinase of the CAMK1 family. Implicated in transcriptional regulation in lymphocytes, neurons and male germ cells. Two alternatively sliced isoforms have been described.

Protein type: Protein kinase, Ser/Thr (non-receptor); Protein kinase, CAMK; EC 2.7.11.17; Kinase, protein; CAMK group; CAMK1 family

Chromosomal Location of Human Ortholog: 5q21.3

Cellular Component: nucleoplasm; nucleolus; cytosol

Molecular Function: calmodulin binding; calmodulin-dependent protein kinase activity; ATP binding

Biological Process: epidermal growth factor receptor signaling pathway; mitochondrion organization and biogenesis; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; organelle organization and biogenesis; regulation of T cell differentiation in the thymus; positive regulation of transcription, DNA-dependent; myeloid dendritic cell differentiation; nucleocytoplasmic transport; signal transduction; protein amino acid phosphorylation; positive regulation of protein export from nucleus; synaptic transmission; regulation of osteoclast differentiation; long-term memory; phospholipase C activation; innate immune response; nerve-nerve synaptic transmission; inflammatory response; myeloid dendritic cell cytokine production