Typical Testing Data/Standard Curve (for reference only)
()
Human Calcium/calmodulin dependent protein kinase type IV (CAMK4) ELISA Kit | CAMK4 elisa kit
Human Calcium/calmodulin dependent protein kinase type IV (CAMK4) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
()
Typical Testing Data/Standard Curve (for reference only)
()
Principle of the Assay: CAMK4 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-CAMK4 antibody and a CAMK4-HRP conjugate. The assay sample and buffer are incubated together with CAMK4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CAMK4 concentration since CAMK4 from samples and CAMK4-HRP conjugate compete for the anti-CAMK4 antibody binding site. Since the number of sites is limited, as more sites are occupied by CAMK4 from the sample, fewer sites are left to bind CAMK4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CAMK4 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The product of this gene belongs to the serine/threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. This enzyme is a multifunctional serine/threonine protein kinase with limited tissue distribution, that has been implicated in transcriptional regulation in lymphocytes, neurons and male germ cells. [provided by RefSeq, Jul 2008]
Uniprot Description
CAMK4: a protein kinase of the CAMK1 family. Implicated in transcriptional regulation in lymphocytes, neurons and male germ cells. Two alternatively sliced isoforms have been described.
Protein type: Protein kinase, Ser/Thr (non-receptor); Protein kinase, CAMK; EC 2.7.11.17; Kinase, protein; CAMK group; CAMK1 family
Chromosomal Location of Human Ortholog: 5q21.3
Cellular Component: nucleoplasm; nucleolus; cytosol
Molecular Function: calmodulin binding; calmodulin-dependent protein kinase activity; ATP binding
Biological Process: epidermal growth factor receptor signaling pathway; mitochondrion organization and biogenesis; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; organelle organization and biogenesis; regulation of T cell differentiation in the thymus; positive regulation of transcription, DNA-dependent; myeloid dendritic cell differentiation; nucleocytoplasmic transport; signal transduction; protein amino acid phosphorylation; positive regulation of protein export from nucleus; synaptic transmission; regulation of osteoclast differentiation; long-term memory; phospholipase C activation; innate immune response; nerve-nerve synaptic transmission; inflammatory response; myeloid dendritic cell cytokine production
Research Articles on CAMK4
Similar Products
Product Notes
The Human CAMK4 camk4 (Catalog #AAA7230332) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7230332 ELISA Kit recognizes Human CAMK4. It is sometimes possible for the material contained within the vial of "Calcium/calmodulin dependent protein kinase type IV (CAMK4), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
