Rabbit anti-Human, Mouse Prkca Polyclonal Antibody | anti-Prkca antibody

Prkca, NT (Prkca, Pkca, Protein kinase C alpha type) (Biotin)

Cat. Num. AAA6337305

• Gene Names: Prkca; Pkca; AI875142
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, ELISA, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Prkca; Polyclonal Antibody; NT (Prkca; Pkca; Protein kinase C alpha type) (Biotin); Protein kinase C alpha type; anti-Prkca antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-Prkca antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; ELISA: 1:1,000; IHC: 1:10-50; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: Mouse Prkca antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of Mouse Prkca.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Mouse Prkca Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the Prkca antibody detected the Prkca protein (arrow).)

Western Blot (WB)

(Mouse Prkca Antibody (N-term) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the Prkca antibody detected the Prkca protein (arrow).)

Flow Cytometry (FC/FACS)

(Mouse Prkca Antibody (N-term) flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)

Immunohistochemistry (IHC)

(Mouse Prkca Antibody (N-term) (AP14277a)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Prkca Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-Prkca antibody

This is a calcium-activated, phospholipid-dependent, serine-and threonine-specific enzyme. May play a role in cell motility by phosphorylating CSPG4 (By similarity). PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.

Product Categories/Family for anti-Prkca antibody

Antibodies; Protein Kinases

References

Yang, L., et al. Biochem. Biophys. Res. Commun. 400(1):16-20(2010). Jin, K., et al. J. Neurosci. 30(36):11902-11916(2010). Peng, H., et al. Pflugers Arch. 460(4):791-802(2010). Wu, M., et al. Dev. Cell 19(1):114-125(2010). Debata, P.R., et al. Biochem. Biophys. Res. Commun. 397(3):401-406(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 164663791
• NCBI GeneID: 18750
• NCBI Accession #: NP_035231.2
• NCBI GenBank Nucleotide #: NM_011101.3
• UniProt Accession #: P20444
• Molecular Weight: 76,852 Da
• NCBI Official Full Name: protein kinase C alpha type
• NCBI Official Synonym Full Names: protein kinase C, alpha
• NCBI Official Symbol: Prkca
• NCBI Official Synonym Symbols: Pkca; AI875142
• NCBI Protein Information: protein kinase C alpha type
• UniProt Protein Name: Protein kinase C alpha type
• Protein Family: Protein kinase
• UniProt Gene Name: Prkca
• UniProt Synonym Gene Names: Pkca; PKC-A; PKC-alpha
• UniProt Entry Name: KPCA_MOUSE

Uniprot Description

PKCA: an AGC kinase of the PKC family. A classical PKC downstream of many mitogenic and receptors. Classical PKCs are calcium-dependent enzymes that are activated by phosphatidylserine, diacylglycerol and phorbol esters. Contains a pseudo-substrate autoinhibitory domain that binds to the catalytic domain preventing its activation in the absence of cofactors or activators.

Protein type: Kinase, protein; Protein kinase, AGC; Oncoprotein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.13; AGC group; PKC family; Alpha subfamily

Cellular Component: apical part of cell; axon; cell soma; cytoplasm; cytosol; dendrite; endoplasmic reticulum; lipid raft; membrane; mitochondrion; neuron projection; nucleus; perinuclear region of cytoplasm; photoreceptor outer segment; plasma membrane; protein complex

Molecular Function: ATP binding; calcium-dependent protein kinase C activity; enzyme binding; kinase activity; metal ion binding; nucleotide binding; protein binding; protein kinase activity; protein kinase C activity; protein serine/threonine kinase activity; transferase activity; zinc ion binding

Biological Process: angiogenesis; apoptosis; cell adhesion; cellular calcium ion homeostasis; central nervous system neuron axonogenesis; chondrocyte differentiation; inactivation of MAPK activity; induction of positive chemotaxis; learning and/or memory; negative regulation of cell proliferation; negative regulation of glucose import; negative regulation of heart contraction; negative regulation of insulin receptor signaling pathway; negative regulation of protein amino acid phosphorylation; negative regulation of protein kinase activity; negative regulation of translation; neutrophil chemotaxis; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; phosphorylation; positive regulation of angiogenesis; positive regulation of blood vessel endothelial cell migration; positive regulation of cell adhesion; positive regulation of cell migration; positive regulation of endothelial cell proliferation; positive regulation of exocytosis; positive regulation of inflammatory response; positive regulation of lipopolysaccharide-mediated signaling pathway; positive regulation of macrophage differentiation; positive regulation of mitotic cell cycle; positive regulation of protein amino acid phosphorylation; positive regulation of smooth muscle cell proliferation; positive regulation of synaptogenesis; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of muscle contraction; regulation of peptidyl-tyrosine phosphorylation; regulation of receptor-mediated endocytosis; regulation of the force of heart contraction; response to estradiol stimulus; response to ethanol; response to reactive oxygen species