Rabbit Rho A + B + C Polyclonal Antibody | anti-RHOC antibody

Rho A + B + C Antibody

Cat. Num. AAA9604427

• Gene Names: RHOC; H9; ARH9; ARHC; RHOH9
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: Rho A + B + C; Polyclonal Antibody; Rho A + B + C Antibody; ARH12; ARHA; H12; ras homolog gene family member A; ras homolog gene family member B; ras homolog gene family member C; Rho cDNA clone 12; RHO12; RHOA; RHOA_HUMAN; rhob; rhoc; RHOH12; Small GTP binding protein RhoA; Transforming protein RhoA; Aplysia ras related homolog 12; H 12; Oncogene RHO H12; Ras homolog family member A; Ras homolog gene family member A; Rho A; RHO H12; Small GTP binding protein Rho A; Transforming protein Rho A; Aplysia RAS-related homolog 6; ARH6; ARHB; H6; MST081; MSTP081; oncogene RHO H6; ras homolog family member B; ras homolog gene family; member B; Rho cDNA clone 6; Rho related GTP binding protein RhoB Precursor; Rho-related GTP-binding protein RhoB; Rhob; RHOB_HUMAN; RHOH6; anti-RHOC antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Rho A + B + C antibody detects endogenous levels of total Rho A + B + C
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 193

• Applicable Applications for anti-RHOC antibody: Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
• Application Notes: WB: 1:1000-3000; IHC: 1:200
• Immunogen: A synthesized peptide derived from human Rho A + B + C
• Subcellular Location: Cell Membrane
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Immunohistochemistry (IHC)

(MBS9604427 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Western Blot (WB)

(Western blot analysis of Rho A + B + C using HUVEC whole cell lysates)

Immunofluorescence (IF)

(MBS9604427 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

Related Product Information for anti-RHOC antibody

Function: Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Serves as a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Regulates apical junction formation in bronchial epithelial cells.
Subunit Structure: Interacts with RTKN (By similarity). Interacts with AKAP13 (PubMed:11546812). Interacts with DIAPH1 (PubMed:15864301). Interacts with PKN2 (PubMed:20974804). Interacts with ROCK1 and ROCK2 (PubMed:8816443). Interacts with ARHGDIA (PubMed:20400958). Interacts with RIPOR1 (PubMed:27807006).
Post-translational Modifications: (Microbial infection) Glycosylated at Tyr-34 by Photorhabdus asymbiotica toxin PAU_02230. Mono-O-GlcNAcylation by PAU_02230 inhibits downstream signaling by an impaired interaction with diverse regulator and effector proteins of Rho and leads to actin disassembly.
Similarity: Belongs to the small GTPase superfamily. Rho family.

NCBI and Uniprot Product Information

• NCBI GI #: 111494248
• NCBI GeneID: 389
• NCBI Accession #: NP_001036143.1
• NCBI GenBank Nucleotide #: NM_001042678.1
• UniProt Accession #: P08134; Q6ICN3; B3KSW1
• Molecular Weight: Observed: 24 kDa; Predicted: 23 kDa
• NCBI Official Full Name: rho-related GTP-binding protein RhoC
• NCBI Official Synonym Full Names: ras homolog family member C
• NCBI Official Symbol: RHOC
• NCBI Official Synonym Symbols: H9; ARH9; ARHC; RHOH9
• NCBI Protein Information: rho-related GTP-binding protein RhoC
• UniProt Protein Name: Rho-related GTP-binding protein RhoC
• Protein Family: Rho-related GTP-binding protein
• UniProt Gene Name: RHOC
• UniProt Synonym Gene Names: ARH9; ARHC; h9

NCBI Description

This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. The protein encoded by this gene is prenylated at its C-terminus, and localizes to the cytoplasm and plasma membrane. It is thought to be important in cell locomotion. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Serves as a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Regulates apical junction formation in bronchial epithelial cells.