Human Calcipressin-2 (RCAN2) ELISA Kit | RCAN2 elisa kit

Human Calcipressin-2 (RCAN2) ELISA Kit

Cat. Num. AAA2802071

• Gene Names: RCAN2; CSP2; RCN2; MCIP2; ZAKI4; ZAKI-4; DSCR1L1
• Reactivity: Human
• Synonyms: Calcipressin-2 (RCAN2); Human Calcipressin-2 (RCAN2) ELISA Kit; RP11-38F19.1; CSP2; DSCR1L1; MCIP2; RCN2; ZAKI-4; ZAKI4; hRCN2; Calcipressin 2 (Thyroid hormone-responsive protein ZAKI-4) (Down syndrome candidate region 1-like 1) (Myocyte-enriched calcineurin int; RCAN2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human RCAN2. No significant cross-reactivity or interference between Human RCAN2 and analogues was observed.

• Assay Type: Sandwich
• Assay: Serum, Plasma, Other biological fluids
• Analysis Method: Quantitive
• Detection Wavelength: 450nm
• Sample Volume: 1-200uL
• Assay Duration: 1-4.5h
• Precision: Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Test Principle: This assay employs a two-site sandwich ELISA to quantitate RCAN2 in samples. An antibody specific for RCAN2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRCAN2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RCAN2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RCAN2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
• Preparation and Storage: Store at 2-8 degree C. Please refer to Instruction Manual.

Related Product Information for RCAN2 elisa kit

Using a differential display of mRNA by PCR, Miyazaki et al. (1996) cloned a cDNA fragment of a thyroid hormone-responsive gene, which they called ZAKI4 (RCAN2), from cultured human skin fibroblasts. Using 5-prime and 3-prime RACE, they isolated a full-length cDNA, which gives rise to 2 mRNA species, both of which encode a protein of 192 amino acids. Sequence analysis of the ZAKI4 protein suggested that the 2 mRNAs arise by alternative polyadenylation. Northern blot analysis revealed the presence of differentially expressed transcripts of 3.4 and 1.4kb. The larger transcript is expressed in skin fibroblasts and skeletal muscle, whereas the smaller transcript is found in heart, brain, and liver but not in placenta, lung, kidney, or pancreas.

NCBI and Uniprot Product Information

• NCBI GI #: 354681997
• NCBI GeneID: 10231
• NCBI Accession #: NP_001238902.1
• NCBI GenBank Nucleotide #: NM_001251973.1
• UniProt Accession #: Q14206; Q5VWF7; Q5VWF8; Q8N116; A6ND07; B3KR46
• Molecular Weight: 27,289 Da
• NCBI Official Full Name: calcipressin-2 isoform 2
• NCBI Official Synonym Full Names: regulator of calcineurin 2
• NCBI Official Symbol: RCAN2
• NCBI Official Synonym Symbols: CSP2; RCN2; MCIP2; ZAKI4; ZAKI-4; DSCR1L1
• NCBI Protein Information: calcipressin-2; Down syndrome candidate region 1-like 1; Down syndrome critical region gene 1-like 1; myocyte-enriched calcineurin-interacting protein 2; thyroid hormone-responsive (skin fibroblasts); thyroid hormone-responsive protein ZAKI-4
• UniProt Protein Name: Calcipressin-2
• Protein Family: Calcipressin
• UniProt Gene Name: RCAN2
• UniProt Synonym Gene Names: DSCR1L1; ZAKI4; MCIP2
• UniProt Entry Name: RCAN2_HUMAN

NCBI Description

This gene encodes a member of the regulator of calcineurin (RCAN) protein family. These proteins play a role in many physiological processes by binding to the catalytic domain of calcineurin A, inhibiting calcineurin-mediated nuclear translocation of the transcription factor NFATC1. Expression of this gene in skin fibroblasts is upregulated by thyroid hormone, and the encoded protein may also play a role in endothelial cell function and angiogenesis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

RCAN2: Inhibits calcineurin-dependent transcriptional responses by binding to the catalytic domain of calcineurin A. Could play a role during central nervous system development. Belongs to the RCAN family. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 6p12.3

Molecular Function: nucleotide binding

Biological Process: calcineurin-NFAT signaling pathway; short-term memory; response to oxidative stress; locomotion during locomotory behavior