Human CA5B ELISA Kit | CA5B elisa kit

Human CA5B (Carbonic Anhydrase VB, Mitochondrial) ELISA Kit

Cat. Num. AAA2511652

• Gene Names: CA5B; CA-VB
• Reactivity: Human
• Synonyms: CA5B; Human CA5B (Carbonic Anhydrase VB; Mitochondrial) ELISA Kit; CA5B elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human CA5B. No significant cross-reactivity or interference between Human CA5B and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human CA5B and all the analogues, therefore, cross reaction may still exist.
• Sequence Length: 317

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: Min: 0.094ng/mL; Max: 10ng/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CA5B elisa kit

Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CA5B in human serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CA5B. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human CA5B and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CA5B, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human CA5B. You can calculate the concentration of Human CA5B in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 6005723
• NCBI GeneID: 11238
• NCBI Accession #: NP_009151.1
• NCBI GenBank Nucleotide #: NM_007220.3
• UniProt Accession #: Q9Y2D0; A8K4T5
• Molecular Weight: 36,434 Da
• NCBI Official Full Name: carbonic anhydrase 5B, mitochondrial
• NCBI Official Synonym Full Names: carbonic anhydrase VB, mitochondrial
• NCBI Official Symbol: CA5B
• NCBI Official Synonym Symbols: CA-VB
• NCBI Protein Information: carbonic anhydrase 5B, mitochondrial; carbonate dehydratase VB; carbonic dehydratase
• UniProt Protein Name: Carbonic anhydrase 5B, mitochondrial
• Protein Family: Carbonic anhydrase
• UniProt Gene Name: CA5B
• UniProt Synonym Gene Names: CA-VB
• UniProt Entry Name: CAH5B_HUMAN

NCBI Description

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA VB is localized in the mitochondria and shows the highest sequence similarity to the other mitochondrial CA, CA VA. It has a wider tissue distribution than CA VA, which is restricted to the liver. The differences in tissue distribution suggest that the two mitochondrial carbonic anhydrases evolved to assume different physiologic roles. [provided by RefSeq, Jul 2008]

Uniprot Description

CA5B: Reversible hydration of carbon dioxide. Belongs to the alpha-carbonic anhydrase family.

Protein type: Lyase; EC 4.2.1.1; Energy Metabolism - nitrogen; Mitochondrial

Chromosomal Location of Human Ortholog: Xp21.1

Cellular Component: mitochondrion; mitochondrial matrix

Molecular Function: carbonate dehydratase activity; zinc ion binding

Biological Process: bicarbonate transport; one-carbon compound metabolic process