Human C-Raf Proto Oncogene Serine/Threonine Protein Kinase (CRAF) ELISA Kit | CRAF elisa kit

Human C-Raf Proto Oncogene Serine/Threonine Protein Kinase (CRAF) ELISA Kit

Cat. Num. AAA2704299

• Gene Names: Raf1; cRaf; Craf1; Raf-1; c-Raf; v-Raf; AA990557; BB129353; 6430402F14Rik; D830050J10Rik
• Reactivity: Human
• Synonyms: C-Raf Proto Oncogene Serine/Threonine Protein Kinase (CRAF); Human C-Raf Proto Oncogene Serine/Threonine Protein Kinase (CRAF) ELISA Kit; RAF1; Raf1; V-raf-1 Murine Leukemia Viral Oncogene Homolog 1; Proto-oncogene c-RAF; CRAF elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of C-Raf Proto Oncogene Serine/Threonine Protein Kinase (CRAF).; No significant cross-reactivity or interference between C-Raf Proto Oncogene Serine/Threonine Protein Kinase (CRAF) and analogues was observed.
• Sequence Length: 668

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.059ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level C-Raf Proto Oncogene Serine/Threonine Protein Kinase (CRAF) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level C-Raf Proto Oncogene Serine/Threonine Protein Kinase (CRAF) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CRAF elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to C-Raf Proto Oncogene Serine/Threonine Protein Kinase (CRAF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to C-Raf Proto Oncogene Serine/Threonine Protein Kinase (CRAF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain C-Raf Proto Oncogene Serine/Threonine Protein Kinase (CRAF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of C-Raf Proto Oncogene Serine/Threonine Protein Kinase (CRAF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 18150196
• NCBI GeneID: 110157
• UniProt Accession #: Q99N57; Q3UR68; Q58E75; Q91WH1; Q99N58; Q9QUU8
• Molecular Weight: 75,151 Da
• NCBI Official Full Name: cRAF, partial
• NCBI Official Synonym Full Names: v-raf-leukemia viral oncogene 1
• NCBI Official Symbol: Raf1
• NCBI Official Synonym Symbols: cRaf; Craf1; Raf-1; c-Raf; v-Raf; AA990557; BB129353; 6430402F14Rik; D830050J10Rik
• NCBI Protein Information: RAF proto-oncogene serine/threonine-protein kinase
• UniProt Protein Name: RAF proto-oncogene serine/threonine-protein kinase
• UniProt Gene Name: Raf1
• UniProt Synonym Gene Names: Craf; cRaf

Uniprot Description

Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation (). Regulates Rho signaling and migration, and is required for normal wound healing.