Human Brucella IgM ELISA Kit | IgM elisa kit
Brucella IgM ELISA
Reactivity
Human
Synonyms
Brucella IgM; Brucella IgM ELISA; IgM elisa kit
Reactivity
Human
Specificity
Brucella IgA, IgG and IgM The Brucella IgA, IgG and IgM ELISA tests were verified in an internal study. The sensitivity and specificity in both cases exceeded 92%.
Application Notes
1 hour (37 degree C) + 30 min. (37 degree C) + 30 min. (37 degree C) = 2 hours total incubation time.
Preparation and Storage
Store at 2-8 degree C
Related Product Information for IgM elisa kit
Intended Uses: The ELISA Brucella IgA, IgG and IgM tests are qualitative immunoassays for the demonstration of human IgA, IgG and IgM antibodies directed against pathogenic Brucella ssp.. The evaluation of individual immunoglobulin classes can be used for the determination of pathogen contact.
Principle of the Assay: The ELISA (Enzyme Linked Immunosorbent Assay) is an immunoassay, which is particularly suited to the determination of antibodies in the field of infectious serology. The reaction is based on the specific interaction of antibodies with their corresponding antigen. The test strips of the microtiter plate are coated with specific antigens of the pathogen of interest. If antibodies in the sample are present, they bind to the fixed antigen. A secondary antibody, which has been conjugated with the enzyme alkaline phosphatase, detects and binds to the immune complex. The colourless substrate pnitrophenylphosphate is then converted into the coloured product p-nitrophenol. The signal intensity of this reaction product is proportional to the concentration of the analyte in the sample and is measured photometrically!!Background/Introduction: Brucella ssp. are gram-negative non-motile bacteria which live as intracellular parasites in a wide spectrum of farm animals. Human infection is primarily caused by Brucella melitensis ("Malta fever"), Brucella abortus ("Morburs Bang") and Brucella suis. The pathogen is transmitted by infected animals (zoonosis), their excrement and contaminated food, particularly unpasteurized dairy products. The disease starts with general symptoms progressing to moderate fever as the acute phase begins, characterized by rising fever in the evenings, hepatomegaly and splenomegaly or swollen lymph nodes. Undulating fever with afebrile intervals are characteristic for Brucella melitensis and Brucella suis infections. Spontaneous healing or transition into a chronic stage with a wide spectrum of symptoms is possible. Multiple organs or organ systems, bones or joints can be affected during the chronic stage. Histologically, characteristic granulomas are observed in infected tissues. Bacterial endocarditis is fatal if it remains untreated. During the late stage of brucellosis, neurologic and even psychiatric manifestations can occur. ELISA test-systems are especially suited for the differential analysis of immunoglobulin classes directed against the virus.
Principle of the Assay: The ELISA (Enzyme Linked Immunosorbent Assay) is an immunoassay, which is particularly suited to the determination of antibodies in the field of infectious serology. The reaction is based on the specific interaction of antibodies with their corresponding antigen. The test strips of the microtiter plate are coated with specific antigens of the pathogen of interest. If antibodies in the sample are present, they bind to the fixed antigen. A secondary antibody, which has been conjugated with the enzyme alkaline phosphatase, detects and binds to the immune complex. The colourless substrate pnitrophenylphosphate is then converted into the coloured product p-nitrophenol. The signal intensity of this reaction product is proportional to the concentration of the analyte in the sample and is measured photometrically!!Background/Introduction: Brucella ssp. are gram-negative non-motile bacteria which live as intracellular parasites in a wide spectrum of farm animals. Human infection is primarily caused by Brucella melitensis ("Malta fever"), Brucella abortus ("Morburs Bang") and Brucella suis. The pathogen is transmitted by infected animals (zoonosis), their excrement and contaminated food, particularly unpasteurized dairy products. The disease starts with general symptoms progressing to moderate fever as the acute phase begins, characterized by rising fever in the evenings, hepatomegaly and splenomegaly or swollen lymph nodes. Undulating fever with afebrile intervals are characteristic for Brucella melitensis and Brucella suis infections. Spontaneous healing or transition into a chronic stage with a wide spectrum of symptoms is possible. Multiple organs or organ systems, bones or joints can be affected during the chronic stage. Histologically, characteristic granulomas are observed in infected tissues. Bacterial endocarditis is fatal if it remains untreated. During the late stage of brucellosis, neurologic and even psychiatric manifestations can occur. ELISA test-systems are especially suited for the differential analysis of immunoglobulin classes directed against the virus.
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Product Notes
The Human IgM (Catalog #AAA490271) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA490271 ELISA Kit recognizes Human IgM. 1 hour (37 degree C) + 30 min. (37 degree C) + 30 min. (37 degree C) = 2 hours total incubation time. Researchers should empirically determine the suitability of the IgM for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Brucella IgM, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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