Human Breast Cancer Anti-Estrogen Resistance 1 (BCAR1) ELISA Kit | BCAR1 elisa kit

Human Breast Cancer Anti-Estrogen Resistance 1 (BCAR1) ELISA Kit

Cat. Num. AAA2706211

• Gene Names: BCAR1; CAS; CAS1; CASS1; CRKAS; P130Cas
• Reactivity: Human
• Synonyms: Breast Cancer Anti-Estrogen Resistance 1 (BCAR1); Human Breast Cancer Anti-Estrogen Resistance 1 (BCAR1) ELISA Kit; CAS; CRKAS; P130Cas; CASS1; Crk-Associated Substrate; Cas Scaffolding Protein Family Member 1; BCAR1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Breast Cancer Anti-Estrogen Resistance 1 (BCAR1).; No significant cross-reactivity or interference between Breast Cancer Anti-Estrogen Resistance 1 (BCAR1) and analogues was observed.
• Sequence Length: 870

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates and Other Biological Fluids
• Detection Range: 78.12-5000pg/mL
• Sensitivity: < 27pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Breast Cancer Anti-Estrogen Resistance 1 (BCAR1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Breast Cancer Anti-Estrogen Resistance 1 (BCAR1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BCAR1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Breast Cancer Anti-Estrogen Resistance 1 (BCAR1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Breast Cancer Anti-Estrogen Resistance 1 (BCAR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Breast Cancer Anti-Estrogen Resistance 1 (BCAR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Breast Cancer Anti-Estrogen Resistance 1 (BCAR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 38566194
• NCBI GeneID: 9564
• Molecular Weight: 93,291 Da
• NCBI Official Full Name: Breast cancer anti-estrogen resistance 1
• NCBI Official Synonym Full Names: BCAR1, Cas family scaffolding protein
• NCBI Official Symbol: BCAR1
• NCBI Official Synonym Symbols: CAS; CAS1; CASS1; CRKAS; P130Cas
• NCBI Protein Information: breast cancer anti-estrogen resistance protein 1
• UniProt Protein Name: Breast cancer anti-estrogen resistance protein 1
• Protein Family: Breast cancer anti-estrogen resistance protein
• UniProt Gene Name: BCAR1
• UniProt Synonym Gene Names: CAS; CASS1; CRKAS

NCBI Description

The protein encoded by this gene is a member of the Crk-associated substrate (CAS) family of scaffold proteins, characterized by the presence of multiple protein-protein interaction domains and many serine and tyrosine phosphorylation sites. The encoded protein contains a Src-homology 3 (SH3) domain, a proline-rich domain, a substrate domain which contains 15 repeat of the YxxP consensus phosphorylation motif for Src family kinases, a serine-rich domain, and a bipartite Src-binding domain, which can bind both SH2 and SH3 domains. This adaptor protein functions in multiple cellular pathways, including in cell motility, apoptosis and cell cycle control. Dysregulation of this gene can have a wide range of effects, affecting different pathways, including cardiac development, vascular smooth muscle cells, liver and kidney function, endothelial migration, and cancer. [provided by RefSeq, Sep 2017]

Uniprot Description

Docking protein which plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion. Implicated in induction of cell migration. Overexpression confers antiestrogen resistance on breast cancer cells.