Human Bovine Serum Albumin (BSA) ELISA Kit | BSA elisa kit

Human Bovine Serum Albumin (BSA) ELISA Kit

Cat. Num. AAA266586

• Reactivity: Human
• Synonyms: Bovine Serum Albumin (BSA); Human Bovine Serum Albumin (BSA) ELISA Kit; BSA elisa kit

• Reactivity: Human
• Specificity: No cross-reaction with other factors.
• Sequence Length: 607

• Samples: Human serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 200 ug/mL-3.12 ug/mL
• Sensitivity: Up to 0.6 ug/mL
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for BSA elisa kit

Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Human BSA monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 3336842
• NCBI GeneID: 280717
• UniProt Accession #: P02769; O02787; P04277; Q3SZR2; A5PJX3
• Molecular Weight: 69,293 Da
• NCBI Official Full Name: bovine serum albumin
• NCBI Official Synonym Full Names: albumin
• NCBI Official Symbol: ALB
• NCBI Protein Information: serum albumin; BSA
• UniProt Protein Name: Serum albumin
• UniProt Gene Name: ALB
• UniProt Entry Name: ALBU_BOVIN

Uniprot Description

albumin: Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc

Protein type: Secreted, signal peptide; Secreted; Carrier

Cellular Component: protein complex; cytoplasm; extracellular region; nucleus

Molecular Function: toxin binding; DNA binding; metal ion binding; chaperone binding; drug binding; oxygen binding; fatty acid binding; pyridoxal phosphate binding

Biological Process: retinal homeostasis; maintenance of mitochondrion localization; transport; hemolysis by symbiont of host red blood cells; cellular response to starvation; negative regulation of apoptosis