Human Bone Morphogenetic Protein 15 (BMP15) ELISA Kit | BMP15 elisa kit

Human Bone Morphogenetic Protein 15 (BMP15) ELISA Kit

Cat. Num. AAA2022519

• Gene Names: BMP15; ODG2; POF4; GDF9B
• Reactivity: Human
• Synonyms: Bone Morphogenetic Protein 15 (BMP15); Human Bone Morphogenetic Protein 15 (BMP15) ELISA Kit; GDF9B; GDF9-B; ODG2; POF4; Growth/differentiation factor 9B; BMP15 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Bone Morphogenetic Protein 15 (BMP15).; No significant cross-reactivity or interference between Bone Morphogenetic Protein 15 (BMP15) and analogues was observed.
• Sequence Length: 392

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
• Detection Range: 31.2-2000pg/mL
• Sensitivity: < 12.7pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bone Morphogenetic Protein 15 (BMP15) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bone Morphogenetic Protein 15 (BMP15) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BMP15 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bone Morphogenetic Protein 15 (BMP15). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Bone Morphogenetic Protein 15 (BMP15). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bone Morphogenetic Protein 15 (BMP15), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Bone Morphogenetic Protein 15 (BMP15) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for BMP15 elisa kit

Signal transduction; Reproductive science; Developmental science; Bone metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 257743454
• NCBI GeneID: 9210
• NCBI Accession #: NP_005439.2
• NCBI GenBank Nucleotide #: NM_005448.2
• UniProt Accession #: O95972; Q17RM6; Q5JST1; Q9UMS1
• Molecular Weight: 45,055 Da
• NCBI Official Full Name: bone morphogenetic protein 15
• NCBI Official Synonym Full Names: bone morphogenetic protein 15
• NCBI Official Symbol: BMP15
• NCBI Official Synonym Symbols: ODG2; POF4; GDF9B
• NCBI Protein Information: bone morphogenetic protein 15
• UniProt Protein Name: Bone morphogenetic protein 15
• Protein Family: Bone morphogenetic protein
• UniProt Gene Name: BMP15
• UniProt Synonym Gene Names: GDF9B; BMP-15; GDF-9B
• UniProt Entry Name: BMP15_HUMAN

NCBI Description

The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. The transforming growth factor-beta superfamily includes large families of growth and differentiation factors. It is thought that this protein may be involved in oocyte maturation and follicular development as a homodimer or by forming heterodimers with a related protein, Gdf9. Defects in this gene are the cause of ovarian dysgenesis 2.[provided by RefSeq, Sep 2009]

Uniprot Description

BMP15: May be involved in follicular development. Oocyte- specific growth/differentiation factor that stimulates folliculogenesis and granulosa cell (GC) growth. Defects in BMP15 are the cause of ovarian dysgenesis type 2 (ODG2); also known as X-linked hypergonadotropic ovarian dysgenesis or hypergonadotropic ovarian failure due to ovarian dysgenesis. Ovarian dysgenesis leads to ovarian failure and accounts for about half of the cases of primary amenorrhea. Defects in BMP15 are the cause of premature ovarian failure type 4 (POF4). An ovarian disorder defined as the cessation of ovarian function under the age of 40 years. It is characterized by oligomenorrhea or amenorrhea, in the presence of elevated levels of serum gonadotropins and low estradiol. Belongs to the TGF-beta family.

Protein type: Secreted, signal peptide; Cytokine; Secreted

Chromosomal Location of Human Ortholog: Xp11.2

Cellular Component: extracellular space; cytoplasm

Molecular Function: growth factor activity; cytokine activity; transforming growth factor beta receptor binding

Biological Process: regulation of apoptosis; BMP signaling pathway; granulosa cell development; ovarian follicle development; regulation of MAPKKK cascade; positive regulation of transcription, DNA-dependent; female gamete generation; cell development

Disease: Ovarian Dysgenesis 2