Typical Testing Data/Standard Curve (for reference only)
Human Bifunctional protein NCOAT (MGEA5) ELISA Kit | MGEA5 elisa kit
Human Bifunctional protein NCOAT (MGEA5) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: MGEA5 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-MGEA5 antibody and an MGEA5-HRP conjugate. The assay sample and buffer are incubated together with MGEA5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MGEA5 concentration since MGEA5 from samples and MGEA5-HRP conjugate compete for the anti-MGEA5 antibody binding site. Since the number of sites is limited, as more sites are occupied by MGEA5 from the sample, fewer sites are left to bind MGEA5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MGEA5 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The dynamic modification of cytoplasmic and nuclear proteins by O-linked N-acetylglucosamine (O-GlcNAc) addition and removal on serine and threonine residues is catalyzed by OGT (MIM 300255), which adds O-GlcNAc, and MGEA5, a glycosidase that removes O-GlcNAc modifications (Gao et al., 2001 [PubMed 11148210]).[supplied by OMIM, Mar 2008]
Uniprot Description
OGA: Cleaves GlcNAc but not GalNAc from glycopeptides. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl- beta-GalNAc or p-nitrophenyl-alpha-GlcNAc. Possesses hyaluronidase activity. Acetylates 'Lys-8' of histone H4 and 'Lys-14' of histone H3. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Acetyltransferase; Hydrolase; EC 3.2.1.169; EC 3.2.1.52
Chromosomal Location of Human Ortholog: 10q24.1-q24.3
Cellular Component: membrane; cytosol; nucleus
Molecular Function: beta-N-acetylglucosaminidase activity; histone acetyltransferase activity; hyalurononglucosaminidase activity
Biological Process: positive regulation of DNA metabolic process; positive regulation of protein complex disassembly; dATP metabolic process; positive regulation of proteolysis; protein deglycosylation; N-acetylglucosamine metabolic process; positive regulation of insulin secretion; positive regulation of growth hormone secretion; positive regulation of glucose import; negative regulation of protein amino acid glycosylation; positive regulation of mitochondrial depolarization; response to steroid hormone stimulus; positive regulation of cell killing; glycoprotein catabolic process; histone acetylation; protein targeting to membrane; aging
Research Articles on MGEA5
Similar Products
Product Notes
The Human MGEA5 mgea5 (Catalog #AAA7213506) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7213506 ELISA Kit recognizes Human MGEA5. It is sometimes possible for the material contained within the vial of "Bifunctional protein NCOAT (MGEA5), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
