Typical Testing Data/Standard Curve
Human beta soluble NSF attachment protein (NAPB) ELISA Kit | NAPB elisa kit
Human beta soluble NSF attachment protein (NAPB) ELISA Kit
Gene Names
NAPB; SNAPB; SNAP-BETA
Reactivity
Human
Synonyms
beta soluble NSF attachment protein (NAPB); Human beta soluble NSF attachment protein (NAPB) ELISA Kit; Human b soluble NSF attachment protein (NAPB) ELISA Kit; NAPB elisa kit
Reactivity
Human
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Sensitivity
1.0 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve
Typical Testing Data/Standard Curve
Related Product Information for NAPB elisa kit
Intended Uses: This NAPB ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human NAPB. This ELISA kit for research use only!
Principle of the Assay||NAPB ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-NAPB antibody and an NAPB-HRP conjugate. The assay sample and buffer are incubated together with NAPB-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NAPB concentration since NAPB from samples and NAPB-HRP conjugate compete for the anti-NAPB antibody binding site. Since the number of sites is limited, as more sites are occupied by NAPB from the sample, fewer sites are left to bind NAPB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NAPB concentration in each sample is interpolated from this standard curve.
Principle of the Assay||NAPB ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-NAPB antibody and an NAPB-HRP conjugate. The assay sample and buffer are incubated together with NAPB-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NAPB concentration since NAPB from samples and NAPB-HRP conjugate compete for the anti-NAPB antibody binding site. Since the number of sites is limited, as more sites are occupied by NAPB from the sample, fewer sites are left to bind NAPB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NAPB concentration in each sample is interpolated from this standard curve.
Product Categories/Family for NAPB elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
23,403 Da
NCBI Official Full Name
beta-soluble NSF attachment protein isoform a
NCBI Official Synonym Full Names
N-ethylmaleimide-sensitive factor attachment protein, beta
NCBI Official Symbol
NAPB
NCBI Official Synonym Symbols
SNAPB; SNAP-BETA
NCBI Protein Information
beta-soluble NSF attachment protein
UniProt Protein Name
Beta-soluble NSF attachment protein
Protein Family
UniProt Gene Name
NAPB
UniProt Synonym Gene Names
SNAPB; SNAP-beta
UniProt Entry Name
SNAB_HUMAN
Uniprot Description
NAPB iso3: Required for vesicular transport between the endoplasmic reticulum and the Golgi apparatus. Belongs to the SNAP family.
Protein type: Endoplasmic reticulum
Chromosomal Location of Human Ortholog: 20p12.3-p11.21
Cellular Component: SNARE complex; vacuolar membrane
Molecular Function: syntaxin binding; soluble NSF attachment protein activity
Biological Process: intracellular protein transport
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Product Notes
The Human NAPB napb (Catalog #AAA7230541) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7230541 ELISA Kit recognizes Human NAPB. It is sometimes possible for the material contained within the vial of "beta soluble NSF attachment protein (NAPB), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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