Typical Testing Data/Standard Curve (for reference only)
Human beta adducin (ADD2) ELISA Kit | ADD2 elisa kit
Human beta adducin (ADD2) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: ADD2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ADD2 antibody and an ADD2-HRP conjugate. The assay sample and buffer are incubated together with ADD2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADD2 concentration since ADD2 from samples and ADD2-HRP conjugate compete for the anti-ADD2 antibody binding site. Since the number of sites is limited, as more sites are occupied by ADD2 from the sample, fewer sites are left to bind ADD2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADD2 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. While adducins alpha and gamma are ubiquitously expressed, the expression of adducin beta is restricted to brain and hematopoietic tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Heterodimers consisting of alpha and gamma subunits have also been described. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jun 2010]
Uniprot Description
ADD2: a cytoskeletal protein that promotes the assembly of the spectrin-actin network. Adducin is a heterodimeric protein that consists of related subunits. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Beta-adducin is restricted to brain and hematopoietic tissues. Three alternatively-spliced isoforms have been described.
Protein type: Motility/polarity/chemotaxis; Cytoskeletal
Chromosomal Location of Human Ortholog: 2p13.3
Cellular Component: F-actin capping protein complex; cytoplasmic membrane-bound vesicle; plasma membrane
Molecular Function: calmodulin binding; actin filament binding; protein homodimerization activity; spectrin binding; protein heterodimerization activity; structural molecule activity; actin binding
Biological Process: actin filament bundle formation; positive regulation of protein binding; hemopoiesis; protein complex assembly; barbed-end actin filament capping; actin cytoskeleton organization and biogenesis
Research Articles on ADD2
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Product Notes
The Human ADD2 add2 (Catalog #AAA7212088) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7212088 ELISA Kit recognizes Human ADD2. It is sometimes possible for the material contained within the vial of "beta adducin (ADD2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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