Human BDNF ELISA Kit | BDNF elisa kit

Human BDNF Quick ELISA Kit

Cat. Num. AAA1753904

• Gene Names: BDNF; ANON2; BULN2
• Reactivity: Human
• Synonyms: BDNF; Human BDNF Quick ELISA Kit; Brain-derived neurotrophic factor; Abrineurin [Cleaved into: BDNF precursor form|ProBDNF]; brain derived neurotrophic factor; BDNF elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human BDNF

• Assay Type: Colorimetric sandwich
• Samples: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA, citrate)
• Detection Range: 31.2pg/ml-2,000pg/ml
• Sensitivity: <15pg/ml
• Capture/Detection Antibodies: Capture antibody- monoclonal antibody from mouse|Detection antibody- polyclonal antibody from goat
• Sample Data: Concentration(pg/ml)|0|31.2|62.5|125|250|500|1000|2000; O.D.|0.014|0.076|0.120|0.255|0.485|1.020|1.955|2.401
• Intra-Assay Precision: Sample|1|2|3|; n|16|16|16|; Mean(pg/ml)|50|234|1124|; Standard deviation|3.6|11.23|55.07|; CV(%)|7.2%|4.8%|4.9%|
• Inter-Assay Precision: Sample|1|2|3|; n|24|24|24; Mean(pg/ml)|48|254|987; Standard deviation|4.17|13.97|63.16; CV(%)|8.7%|5.5%|6.4%
• Reproducibility: To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.; Lots: Lot 1 (pg/ml)|Lot 2 (pg/ml)|Lot 3 (pg/ml)|Lot 4 (pg/ml)|Mean (pg/ml)|Standard Deviation|CV (%); Sample 1: 50|55|52|52|52|2.7|5.2%; Sample 2: 234|198|232|224|169|11.32|6.7%; Sample 3: 1124|1121|884|1142|1067|76.82|7.2%; *number of samples for each test n=16.
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: NS0; Immunogen sequence: H129-R247
• Anticoagulant: heparin, EDTA or citrate
• Benefits: The Quick ELISA kits, assay takes less than 1.5 hours. Detect Human BDNF with <15pg/ml sensitivity. Format: 96-well plate with removable strips.; This is a TMB colorimetric sandwich ELISA kit with short assay time and quick experiment set up.
• Principle: Human BDNF Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human BDNF with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for BDNF. The detection antibody is a HRP linked goat polyclonal antibody specific for BDNF. The kit contains recombinant Human BDNF with immunogen: Expression system for standard: NS0; Immunogen sequence: H129-R247. The kit is analytically validated with ready to use reagents.; ; To measure Human BDNF, add standards and samples to the wells, then add HRP linked detection antibody. Wash the wells with PBS or TBS buffer, and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human BDNF in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human BDNF in the sample.
• Assay Procedure Step 1: 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.25pg/ml
• TMB Incubation Time: 15
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice.)

Testing Data

Related Product Information for BDNF elisa kit

Brain-derived neurotrophic factor(BDNF) is a prosurvival factor induced by cortical neurons that is necessary for survival of striatal neurons in the brain. It is a secreted protein with the molecular weight of 27. 8kDa, consisting of 247 amino acids. It is known to promote neuronal survival and differentiation. BDNF shares substantial amino acid sequence identity with nerve growth factor(NGF). BDNF and neurotrophin-3(NT-3) are two recently cloned neurotrophic factors that are homologous to NGF. mRNA products of the BDNF and NT-3 genes are detected in the adult human brain, suggesting that these proteins are involved in the maintenance of the adult nervous system. BDNF and other neurotrophins are critically involved in long-term potentiation(LTP). BDNF-mediated LTP is induced postsynaptically. BDNF has trophic effects on serotonergic(5-HT) neurons in the central nervous system. BDNF has an essential maintenance function in the regulation of anxiety-related behavior and in food intake through central mediators in both the basal and fasted state. It plays a role in treating breathing disorders such as respiratory insufficiency after spinal injury. The mature form of BDNF is identical in all mammals examined, and the gene encoding human BDNF to chromosome 11, band p13.

Product Categories/Family for BDNF elisa kit

ELISA kits

NCBI and Uniprot Product Information

• NCBI GI #: 114900
• NCBI GeneID: 627
• UniProt Accession #: P23560; Q598Q1; Q6DN19; Q6YNR2; Q6YNR3; Q9BYY7; Q9UC24; A7LA85; A7LA92; D3DQZ2
• Molecular Weight: 247
• NCBI Official Full Name: Brain-derived neurotrophic factor
• NCBI Official Synonym Full Names: brain-derived neurotrophic factor
• NCBI Official Symbol: BDNF
• NCBI Official Synonym Symbols: ANON2; BULN2
• NCBI Protein Information: brain-derived neurotrophic factor; abrineurin; neurotrophin
• UniProt Protein Name: Brain-derived neurotrophic factor
• Protein Family: BDNF/NT-3 growth factors receptor
• UniProt Gene Name: BDNF
• UniProt Synonym Gene Names: BDNF
• UniProt Entry Name: BDNF_HUMAN

NCBI Description

The protein encoded by this gene is a member of the nerve growth factor family. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of this gene is reduced in both Alzheimer's and Huntington disease patients. This gene may play a role in the regulation of stress response and in the biology of mood disorders. Multiple transcript variants encoding distinct isoforms have been described for this gene. [provided by RefSeq, Jan 2009]

Uniprot Description

BDNF: During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases. Belongs to the NGF-beta family. 5 isoforms of the human protein are produced by alternative promoter.

Protein type: Secreted, signal peptide; Cytokine; Cell development/differentiation; Secreted

Chromosomal Location of Human Ortholog: 11p13

Cellular Component: extracellular space; synaptic vesicle; mitochondrial crista; perinuclear region of cytoplasm; cytoplasmic membrane-bound vesicle; cytoplasm; dendrite; extracellular region; terminal button; perikaryon

Molecular Function: growth factor activity; neurotrophin TRKB receptor binding

Biological Process: circadian rhythm; axon guidance; mechanoreceptor differentiation; behavioral fear response; mitochondrial electron transport, NADH to ubiquinone; regulation of neuron differentiation; axon extension; response to hormone stimulus; positive regulation of long-term neuronal synaptic plasticity; response to vitamin A; negative regulation of neuroblast proliferation; ureteric bud development; dendrite development; regulation of retinal cell programmed cell death; feeding behavior; negative regulation of neuron apoptosis; response to electrical stimulus; negative regulation of synaptic transmission, GABAergic; inner ear development; response to food; nervous system development; chronic inflammatory response; response to light intensity; neuron recognition; learning; regulation of short-term neuronal synaptic plasticity; regulation of axon extension; negative regulation of striated muscle development; positive regulation of peptidyl-serine phosphorylation; positive regulation of synaptogenesis; axon target recognition; response to hyperoxia; glutamate secretion; response to hypoxia; response to fluoxetine; nerve development; response to activity; regulation of excitatory postsynaptic membrane potential; positive regulation of neuron differentiation; neurite morphogenesis; transmembrane receptor protein tyrosine kinase signaling pathway; gamma-aminobutyric acid signaling pathway

Disease: Bulimia Nervosa, Susceptibility To, 1; Obsessive-compulsive Disorder; Bulimia Nervosa, Susceptibility To, 2; Central Hypoventilation Syndrome, Congenital