Human BCAR3 ELISA Kit | BCAR3 elisa kit

BCAR3 Colorimetric Cell-Based ELISA Kit

Cat. Num. AAA9500683

• Gene Names: BCAR3; NSP2; AND-34; SH2D3B
• Reactivity: Human
• Synonyms: BCAR3; BCAR3 Colorimetric Cell-Based ELISA Kit; Breast cancer anti-estrogen resistance protein 3; SH2 domain-containing protein 3B; Novel SH2-containing protein 2; NSP2; SH2D3B; BCAR3 elisa kit

• Reactivity: Human
• Form/Format: One 96-Well Plate
• Sequence Length: 825

• Samples: Researchers plate their cell line of choice
• Assay Type: Non-Phospho Cell-Based ELISA
• Sensitivity: >=5000 cells/well
• Detection Method: Colorimetric 450 nm
• Preparation and Storage: Store at 4 degree C.

Product Categories/Family for BCAR3 elisa kit

Cell Based ELISA Kit

NCBI and Uniprot Product Information

• NCBI GI #: 387157906
• NCBI GeneID: 8412
• NCBI Accession #: NP_001248337.1
• NCBI GenBank Nucleotide #: NM_001261408.1
• UniProt Accession #: O75815; Q5TEW3; Q6UW40; Q9BR50; D3DT43
• Molecular Weight: 92 kDa
• NCBI Official Full Name: breast cancer anti-estrogen resistance protein 3 isoform 1
• NCBI Official Synonym Full Names: BCAR3, NSP family adaptor protein
• NCBI Official Symbol: BCAR3
• NCBI Official Synonym Symbols: NSP2; AND-34; SH2D3B
• NCBI Protein Information: breast cancer anti-estrogen resistance protein 3
• UniProt Protein Name: Breast cancer anti-estrogen resistance protein 3
• Protein Family: Breast cancer anti-estrogen resistance protein
• UniProt Gene Name: BCAR3
• UniProt Synonym Gene Names: NSP2; SH2D3B

NCBI Description

Breast tumors are initially dependent on estrogens for growth and progression and can be inhibited by anti-estrogens such as tamoxifen. However, breast cancers progress to become anti-estrogen resistant. Breast cancer anti-estrogen resistance gene 3 was identified in the search for genes involved in the development of estrogen resistance. The gene encodes a component of intracellular signal transduction that causes estrogen-independent proliferation in human breast cancer cells. The protein contains a putative src homology 2 (SH2) domain, a hall mark of cellular tyrosine kinase signaling molecules, and is partly homologous to the cell division cycle protein CDC48. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012]

Uniprot Description

BCAR3: a ubiquitously expressed regulator of R-Ras activity that has been implicated in breast cancer. A GDP exchange factor whose overexpression confers antiestrogen resistance, activates Rac, PAK1, and the cyclin D1. Can accelerate the degradation of IRS-1, thus regulating signaling through the insulin receptor. When overexpressed, it confers anti-estrogen resistance in breast cancer cell lines. May also be regulated by cellular adhesion to extracellular matrix proteins. Up-regulated by interleukin-1-alpha and TNF-beta in thymus cortical reticular cell lines (mouse).Two alternatively spliced human isoforms have been described.

Protein type: Adaptor/scaffold; GEF; GEF, Ras; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 1p22.1

Cellular Component: intracellular

Molecular Function: guanyl-nucleotide exchange factor activity; protein binding; SH3/SH2 adaptor activity

Biological Process: lens morphogenesis in camera-type eye; positive regulation of peptidyl-serine phosphorylation; positive regulation of signal transduction; regulation of molecular function; response to drug; signal transduction; small GTPase mediated signal transduction