Typical Testing Data/Standard Curve (for reference only)
Human B cell lymphoma/leukemia 11B (BCL11B) ELISA Kit | BCL11B elisa kit
Human B cell lymphoma/leukemia 11B (BCL11B) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: BCAT2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-BCAT2 antibody and an BCAT2-HRP conjugate. The assay sample and buffer are incubated together with BCAT2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BCAT2 concentration since BCAT2 from samples and BCAT2-HRP conjugate compete for the anti-BCAT2 antibody binding site. Since the number of sites is limited, as more sites are occupied by BCAT2 from the sample, fewer sites are left to bind BCAT2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BCAT2 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a C2H2-type zinc finger protein and is closely related to BCL11A, a gene whose translocation may be associated with B-cell malignancies. Although the specific function of this gene has not been determined, the encoded protein is known to be a transcriptional repressor, and is regulated by the NURD nucleosome remodeling and histone deacetylase complex. Four alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Aug 2013]
Uniprot Description
Bcl-11B: Tumor-suppressor protein involved in T-cell lymphomas. May function on the P53-signaling pathway. May be a key regulator of both differentiation and survival during thymocyte development. Repress transcription through direct, TFCOUP2-independent binding to a GC-rich response element. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: C2H2-type zinc finger protein; Nuclear receptor co-regulator
Chromosomal Location of Human Ortholog: 14q32.2
Cellular Component: nucleus
Molecular Function: protein binding; metal ion binding
Biological Process: positive T cell selection; post-embryonic camera-type eye development; regulation of lipid metabolic process; thymus development; transcription, DNA-dependent; regulation of neuron differentiation; T cell differentiation in the thymus; alpha-beta T cell differentiation; odontogenesis of dentine-containing teeth; negative regulation of cell proliferation; striatal medium spiny neuron differentiation; commitment of a neuronal cell to a specific type of neuron in the forebrain; T cell receptor V(D)J recombination; positive regulation of transcription from RNA polymerase II promoter; negative regulation of apoptosis
Research Articles on BCL11B
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Product Notes
The Human BCL11B bcl11b (Catalog #AAA7203011) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7203011 ELISA Kit recognizes Human BCL11B. It is sometimes possible for the material contained within the vial of "B cell lymphoma/leukemia 11B (BCL11B), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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