Typical Testing Data/Standard Curve
Human ATP sensitive inward rectifier potassium channel 10 (KCNJ10) ELISA Kit | KCNJ10 elisa kit
Human ATP sensitive inward rectifier potassium channel 10 (KCNJ10) ELISA Kit
Typical Testing Data/Standard Curve
Typical Testing Data/Standard Curve
Principle of the Assay||KCNJ10 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-KCNJ10 antibody and an KCNJ10-HRP conjugate. The assay sample and buffer are incubated together with KCNJ10-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the KCNJ10 concentration since KCNJ10 from samples and KCNJ10-HRP conjugate compete for the anti-KCNJ10 antibody binding site. Since the number of sites is limited, as more sites are occupied by KCNJ10 from the sample, fewer sites are left to bind KCNJ10-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The KCNJ10 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the inward rectifier-type potassium channel family, characterized by having a greater tendency to allow potassium to flow into, rather than out of, a cell. The encoded protein may form a heterodimer with another potassium channel protein and may be responsible for the potassium buffering action of glial cells in the brain. Mutations in this gene have been associated with seizure susceptibility of common idiopathic generalized epilepsy syndromes. [provided by RefSeq, Jul 2008]
Uniprot Description
Kir4.1: May be responsible for potassium buffering action of glial cells in the brain. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium and cesium. Defects in KCNJ10 are the cause of seizures, sensorineural deafness, ataxia, mental retardation, and electrolyte imbalance (SESAMES). A complex disorder characterized by generalized seizures with onset in infancy, delayed psychomotor development, ataxia, sensorineural hearing loss, hypokalemia, metabolic alkalosis, and hypomagnesemia. Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ10 subfamily.
Protein type: Membrane protein, integral; Channel, ligand-gated; Membrane protein, multi-pass; Channel, potassium
Chromosomal Location of Human Ortholog: 1q23.2
Cellular Component: microvillus; integral to plasma membrane; basolateral plasma membrane; apical plasma membrane; plasma membrane
Molecular Function: identical protein binding; protein binding; ATP-activated inward rectifier potassium channel activity; ATP binding; receptor binding
Biological Process: regulation of long-term neuronal synaptic plasticity; myelination in the central nervous system; response to glucocorticoid stimulus; membrane hyperpolarization; response to blue light; glutamate uptake during transmission of nerve impulse; response to mineralocorticoid stimulus; adult walking behavior; protein homotetramerization; synaptic transmission; visual perception; potassium ion import; optic nerve development; regulation of resting membrane potential; regulation of sensory perception of pain; inflammatory response; potassium ion homeostasis; potassium ion transport
Disease: Pendred Syndrome; Seizures, Sensorineural Deafness, Ataxia, Mental Retardation, And Electrolyte Imbalance; Deafness, Autosomal Recessive 4, With Enlarged Vestibular Aqueduct
Research Articles on KCNJ10
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Product Notes
The Human KCNJ10 kcnj10 (Catalog #AAA7227647) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7227647 ELISA Kit recognizes Human KCNJ10. It is sometimes possible for the material contained within the vial of "ATP sensitive inward rectifier potassium channel 10 (KCNJ10), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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