Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human ATP-dependent RNA helicase DDX25 (DDX25) Standard Curve.)
Human ATP-Dependent RNA Helicase DDX25 (DDX25) ELISA Kit | DDX25 elisa kit
Human ATP-Dependent RNA Helicase DDX25 (DDX25) ELISA Kit
Gene Names
DDX25; GRTH
Reactivity
Human
Synonyms
ATP-Dependent RNA Helicase DDX25 (DDX25); Human ATP-Dependent RNA Helicase DDX25 (DDX25) ELISA Kit; DDX25; GRTH; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 25; gonadotropin-regulated testicular RNA helicase; DDX25 elisa kit
Reactivity
Human
Specificity
Human ATP-dependent RNA helicase DDX25 (DDX25) ELISA Kit has high sensitivity and excellent specificity for detection of Human DDX25. No significant cross-reactivity or interference between Human DDX25 and analogues was observed.
Sequence Length
483
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human ATP-dependent RNA helicase DDX25 (DDX25) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human ATP-dependent RNA helicase DDX25 (DDX25) Standard Curve.)
Related Product Information for DDX25 elisa kit
Description: This Human ATP-dependent RNA helicase DDX25 (DDX25) ELISA Kit employs a two-site sandwich ELISA to quantitate DDX25 in samples. An antibody specific for DDX25 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DDX25 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DDX25 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DDX25 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX25 encodes a member of this family. The encoded protein is a gonadotropin-regulated and developmentally expressed testicular RNA helicase. It may serve to maintain testicular functions related to steroidogenesis and spermatogenesis.
Background: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX25 encodes a member of this family. The encoded protein is a gonadotropin-regulated and developmentally expressed testicular RNA helicase. It may serve to maintain testicular functions related to steroidogenesis and spermatogenesis.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
ATP-dependent RNA helicase DDX25 isoform 1
NCBI Official Synonym Full Names
DEAD-box helicase 25
NCBI Official Symbol
DDX25
NCBI Official Synonym Symbols
GRTH
NCBI Protein Information
ATP-dependent RNA helicase DDX25
UniProt Protein Name
ATP-dependent RNA helicase DDX25
Protein Family
UniProt Gene Name
DDX25
UniProt Synonym Gene Names
GRTH
UniProt Entry Name
DDX25_HUMAN
NCBI Description
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a member of this family. The encoded protein is a gonadotropin-regulated and developmentally expressed testicular RNA helicase. It may serve to maintain testicular functions related to steroidogenesis and spermatogenesis. [provided by RefSeq, Jul 2008]
Research Articles on DDX25
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Product Notes
The Human DDX25 ddx25 (Catalog #AAA9715784) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715784 ELISA Kit recognizes Human DDX25. It is sometimes possible for the material contained within the vial of "ATP-Dependent RNA Helicase DDX25 (DDX25), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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