Human ARL1 ELISA Kit | ARL1 elisa kit

Human ARL1(Aldose Reductase Like Protein 1)ELISA Kit

Cat. Num. AAA2515810

• Reactivity: Human
• Synonyms: ARL1; Human ARL1(Aldose Reductase Like Protein 1)ELISA Kit; ARL1 elisa kit

• Reactivity: Human
• Specificity: This kit recognizes natural and recombinantHumanARL1. No significant cross-reactivity or interference between HumanARL1 and analogues was observed.
• Sequence Length: 183

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 78.125-5000pg/mL
• Sensitivity: Min: 46.875pg/mL; Max: 5000pg/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ARL1 elisa kit

Intended Uses: This ELISA kit applies to the invitro quantitative determination of HumanARL1concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ARL1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ARL1and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ARL1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of ARL1.You can calculate the concentration of ARL1in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 536502
• UniProt Accession #: P38116; D6VQG0
• Molecular Weight: 20,434 Da
• NCBI Official Full Name: ARL1
• UniProt Protein Name: ADP-ribosylation factor-like protein 1
• Protein Family: ARL14 effector protein
• UniProt Gene Name: ARL1
• UniProt Synonym Gene Names: ARF3
• UniProt Entry Name: ARL1_YEAST

Uniprot Description

ARL4A: Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). GTP-binding protein that does not act as an allosteric activator of the cholera toxin catalytic subunit. Recruits CYTH1, CYTH2, CYTH3 and CYTH4 to the plasma membrane in GDP-bound form. Belongs to the small GTPase superfamily. Arf family.

Protein type: G protein, monomeric, ARF; G protein, monomeric; Nucleolus

Cellular Component: cytoplasm; plasma membrane; nucleolus; nucleus

Molecular Function: GTP binding

Biological Process: small GTPase mediated signal transduction; brown fat cell differentiation