Human ARID1A (AT-Rich Interactive Domain-containing Protein 1A) ELISA Kit | ARID1A elisa kit

Human ARID1A (AT-Rich Interactive Domain-containing Protein 1A) ELISA Kit

Cat. Num. AAA2540332

• Gene Names: Arid1a; Osa1; BAF250a; Smarcf1; 1110030E03Rik
• Reactivity: Human
• Synonyms: ARID1A (AT-Rich Interactive Domain-containing Protein 1A); Human ARID1A (AT-Rich Interactive Domain-containing Protein 1A) ELISA Kit; ARID1A elisa kit

• Reactivity: Human
• Specificity: This kit recognizes Human ARID1A in samples. No significant cross-reactivity or interference between Human ARID1A and analogues was observed.
• Sequence Length: 2283

• Samples: Serum, plasma and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.78-50ng/mL
• Sensitivity: 0.47ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human ARID1A were tested 20 times on one plate, respectively.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human ARID1A were tested on 3 different plates, 20 replicates in each plate.
• Preparation and Storage: Store at 4 degree C.

Related Product Information for ARID1A elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human ARID1A concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ARID1A. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ARID1A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ARID1A, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human ARID1A. You can calculate the concentration of Human ARID1A in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 124249109
• NCBI GeneID: 93760
• NCBI Accession #: NP_001074288.1
• NCBI GenBank Nucleotide #: NM_001080819.1
• Molecular Weight: 206,400 Da
• NCBI Official Full Name: AT-rich interactive domain-containing protein 1A
• NCBI Official Synonym Full Names: AT rich interactive domain 1A (SWI-like)
• NCBI Official Symbol: Arid1a
• NCBI Official Synonym Symbols: Osa1; BAF250a; Smarcf1; 1110030E03Rik
• NCBI Protein Information: AT-rich interactive domain-containing protein 1A
• UniProt Protein Name: AT-rich interactive domain-containing protein 1A
• Protein Family: AT-rich interactive domain-containing protein
• UniProt Gene Name: Arid1a
• UniProt Synonym Gene Names: Baf250; Baf250a; Osa1; Smarcf1; ARID domain-containing protein 1A; BAF250; BAF250A
• UniProt Entry Name: ARI1A_MOUSE

Uniprot Description

ARID1A: Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Binds DNA non-specifically. Also involved in vitamin D- coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR- mediated transrepression of the CYP27B1 gene. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. Defects in ARID1A are the cause of mental retardation autosomal dominant type 14 (MRD14). A disease characterized by multiple congenital anomalies and mental retardation. Mental retardation is defined by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. MRD14 patients manifest developmental delay, abnormal corpus callosum, absent/hypoplastic fifth finger/toenails, sparse scalp hair, long eyelashes, and a coarse facial appearance with wide mouth, thick lips, and abnormal ears. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Nuclear receptor co-regulator

Cellular Component: nuclear chromatin; nucleoplasm; SWI/SNF complex

Molecular Function: DNA binding; ligand-dependent nuclear receptor binding; nucleosome binding; protein binding

Biological Process: androgen receptor signaling pathway; ATP-dependent chromatin remodeling; cardiac muscle cell differentiation; chromatin remodeling; establishment and/or maintenance of chromatin architecture; estrogen receptor signaling pathway; forebrain development; glucocorticoid receptor signaling pathway; maintenance of chromatin silencing; negative regulation of transcription from RNA polymerase II promoter; neural tube closure; nucleosome disassembly; positive regulation of transcription, DNA-dependent