Human Arginase, Arg ELISA Kit | Arg elisa kit

Human Arginase, Arg ELISA Kit

Cat. Num. AAA162536

• Reactivity: Human
• Synonyms: Arginase; Arg; Human Arginase; Arg ELISA Kit; Arg elisa kit

• Reactivity: Human
• Sequence Length: 322

• Samples: Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 1ng/ml - 400ng/ml
• Sensitivity: 0.52ng/ml
• Intra-Assay: CV<8%
• Inter-Assay: CV<10%
• Preparation and Storage: Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Arg elisa kit

Intended Uses: This sandwich kit is for the accurate quantitative detection of human Arginase (also known as Arg) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human Arg antibody. Arg present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human Arg Antibody is added and binds to Arg in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated Arg antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human Arg. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 1197498
• NCBI GeneID: 383
• UniProt Accession #: P05089; Q5JWT5; Q5JWT6; Q8TE72; Q9BS50; A6NEA0
• Molecular Weight: 35,664 Da
• NCBI Official Full Name: arginase
• NCBI Official Synonym Full Names: arginase 1
• NCBI Official Symbol: ARG1
• NCBI Protein Information: arginase-1
• UniProt Protein Name: Arginase-1
• UniProt Gene Name: ARG1

NCBI Description

Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by this gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Uniprot Description

Key element of the urea cycle converting L-arginine to urea and L-ornithine, which is further metabolized into metabolites proline and polyamides that drive collagen synthesis and bioenergetic pathways critical for cell proliferation, respectively; the urea cycle takes place primarily in the liver and, to a lesser extent, in the kidneys.