Human Arachidonic Acid, AA ELISA Kit | AA elisa kit
Human Arachidonic Acid, AA ELISA Kit
Reactivity
Human
Synonyms
Arachidonic Acid; AA; Human Arachidonic Acid; AA ELISA Kit; AA elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human 11-Deoxycortisol. No significant cross-reactivity or interference between Human 11-Deoxycortisol and analogues was observed.
Sequence Length
86
Samples
Serum, Plasma And Other Biological Fluids
Assay Type
Competitive
Detection Range
1.25 pmol/mL-20 pmol/mL
Sensitivity
0.625 pmol/mL (mean of 6 independent assays).
Intra-assay Precision
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Related Product Information for AA elisa kit
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 11-Deoxycortisol. Standards or samples are added to the appropriate microtiter plate wells with biotin-conjugated 11-Deoxycortisol. A competitive inhibition reaction is launched between 11-Deoxycortisol (Standards or samples) and biotin-conjugated 11-Deoxycortisol with the pre-coated antibody specific for 11-Deoxycortisol. The more amount of 11-Deoxycortisol in samples, the less antibody bound by biotin-conjugated 11-Deoxycortisol. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of 11-Deoxycortisol in the sample. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
Uniprot Description
Function: Predominant enzyme isoform responsible for arachidonic acid epoxidation in the rat kidney. Catalyzes the NADPH-dependent metabolism of arachidonic acid to a mixture of 5,6-, 8,9-, 11,12-, and 14,15-epoxyeicosatrienoic acids (EETS) as the only oxygenation products. Low rate of lauric acid hydroxylation at the (omega-1)-position.
Catalytic activity: RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.
Cofactor: Heme group
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Product Notes
The Human AA cyp2c23 (Catalog #AAA703581) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA703581 ELISA Kit recognizes Human AA. It is sometimes possible for the material contained within the vial of "Arachidonic Acid, AA, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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