Typical Testing Data/Standard Curve (for reference only)
Human Aquaporin 4, AQP-4 ELISA Kit | AQP-4 elisa kit
Human Aquaporin 4, AQP-4 ELISA Kit
Gene Names
Aqp4; WCH4
Reactivity
Human
Synonyms
Aquaporin 4; AQP-4; Human Aquaporin 4; AQP-4 ELISA Kit; AQP-4 elisa kit
Reactivity
Human
Sequence Length
323
Samples
Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
Assay Type
Quantitative Sandwich
Detection Range
0.05ng/ml - 20ng/ml
Sensitivity
0.022ng/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for AQP-4 elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of Human Aquaporin 4 (also known as AQP-4) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human AQP-4 antibody. AQP-4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human AQP-4 Antibody is added and binds to AQP-4 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated AQP-4 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human AQP-4. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human AQP-4 antibody. AQP-4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human AQP-4 Antibody is added and binds to AQP-4 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated AQP-4 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human AQP-4. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
32,088 Da
NCBI Official Full Name
aquaporin-4 isoform M1
NCBI Official Synonym Full Names
aquaporin 4
NCBI Official Symbol
Aqp4
NCBI Official Synonym Symbols
WCH4
NCBI Protein Information
aquaporin-4
UniProt Protein Name
Aquaporin-4
UniProt Gene Name
Aqp4
UniProt Synonym Gene Names
AQP-4; MIWC
NCBI Description
This gene encodes a member of the aquaporin family of intrinsic membrane proteins that function as water-selective channels in the plasma membranes of many cells. This protein is the predominant aquaporin found in brain and has an important role in brain water homeostasis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. A recent study provided evidence for translational readthrough in this gene and expression of an additional C-terminally extended isoform via the use of an alternative in-frame translation termination codon. [provided by RefSeq, Dec 2015]
Uniprot Description
Forms a water-specific channel. Osmoreceptor which regulates body water balance and mediates water flow within the central nervous system.
Research Articles on AQP-4
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Product Notes
The Human AQP-4 aqp4 (Catalog #AAA162856) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA162856 ELISA Kit recognizes Human AQP-4. It is sometimes possible for the material contained within the vial of "Aquaporin 4, AQP-4, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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