Human Aquaporin-2 (AQP2) ELISA Kit | AQP2 elisa kit

Human Aquaporin-2 (AQP2) ELISA Kit

Cat. Num. AAA262830

• Gene Names: AQP2; AQP-CD; WCH-CD
• Reactivity: Human
• Synonyms: Aquaporin-2 (AQP2); Human Aquaporin-2 (AQP2) ELISA Kit; AQP2 elisa kit

• Reactivity: Human
• Sequence Length: 271

• Samples: Human serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 10 ng/mL-0.156 ng/mL
• Sensitivity: Up to 0.05 ng/mL
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for AQP2 elisa kit

Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Human AQP2 monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 4502179
• NCBI GeneID: 359
• NCBI Accession #: NP_000477.1
• NCBI GenBank Nucleotide #: NM_000486.5
• Molecular Weight: 28,837 Da
• NCBI Official Full Name: aquaporin-2
• NCBI Official Synonym Full Names: aquaporin 2 (collecting duct)
• NCBI Official Symbol: AQP2
• NCBI Official Synonym Symbols: AQP-CD; WCH-CD
• NCBI Protein Information: aquaporin-2; AQP-2; aquaporin-CD; ADH water channel; water-channel aquaporin 2; collecting duct water channel protein; water channel protein for renal collecting duct
• UniProt Protein Name: Aquaporin-2
• UniProt Gene Name: AQP2
• UniProt Synonym Gene Names: AQP-2; AQP-CD
• UniProt Entry Name: AQP2_HUMAN

NCBI Description

This gene encodes a water channel protein located in the kidney collecting tubule. It belongs to the MIP/aquaporin family, some members of which are clustered together on chromosome 12q13. Mutations in this gene have been linked to autosomal dominant and recessive forms of nephrogenic diabetes insipidus. [provided by RefSeq, Oct 2008]

Uniprot Description

AQP2: Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Expressed in renal collecting tubules. Belongs to the MIP/aquaporin (TC 1.A.8) family.

Protein type: Transporter; Transporter, aquaporin family; Membrane protein, multi-pass; Membrane protein, integral; Channel, misc.

Chromosomal Location of Human Ortholog: 12q12-q13

Cellular Component: recycling endosome; protein complex; rough endoplasmic reticulum; membrane; clathrin-coated vesicle; lysosome; basolateral plasma membrane; integral to plasma membrane; early endosome; apical plasma membrane; plasma membrane; trans-Golgi network

Molecular Function: water transporter activity; glycerol transmembrane transporter activity; water channel activity; actin binding; glycerol channel activity; PDZ domain binding

Biological Process: glycerol transport; actin filament depolymerization; response to glucagon stimulus; cellular water homeostasis; apoptosis; water transport; response to lithium ion; response to lipopolysaccharide; cellular response to water deprivation; female pregnancy; excretion; response to salt stress; response to starvation; renal water transport; hyperosmotic response; renal water homeostasis; positive regulation of calcium ion transport; response to calcium ion; transmembrane transport; aging

Disease: Diabetes Insipidus, Nephrogenic, Autosomal