Typical Testing Data/Standard Curve (for reference only)
Human Apolipoprotein L domain containing protein 1 (APOLD1) ELISA Kit | APOLD1 elisa kit
Human Apolipoprotein L domain containing protein 1 (APOLD1) ELISA Kit
Gene Names
APOLD1; VERGE
Reactivity
Human
Synonyms
Apolipoprotein L domain containing protein 1 (APOLD1); Human Apolipoprotein L domain containing protein 1 (APOLD1) ELISA Kit; APOLD1 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of APOLD1. No significant cross-reactivity or interference between APOLD1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between APOLD1 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
1.0 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for APOLD1 elisa kit
Intended Uses: This APOLD1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human APOLD1. This ELISA kit for research use only!
Principle of the Assay: APOLD1 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-APOLD1 antibody and an APOLD1-HRP conjugate. The assay sample and buffer are incubated together with APOLD1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the APOLD1 concentration since APOLD1 from samples and APOLD1-HRP conjugate compete for the anti-APOLD1 antibody binding site. Since the number of sites is limited, as more sites are occupied by APOLD1 from the sample, fewer sites are left to bind APOLD1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The APOLD1 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: APOLD1 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-APOLD1 antibody and an APOLD1-HRP conjugate. The assay sample and buffer are incubated together with APOLD1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the APOLD1 concentration since APOLD1 from samples and APOLD1-HRP conjugate compete for the anti-APOLD1 antibody binding site. Since the number of sites is limited, as more sites are occupied by APOLD1 from the sample, fewer sites are left to bind APOLD1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The APOLD1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
27,063 Da
NCBI Official Full Name
apolipoprotein L domain-containing protein 1 isoform 1
NCBI Official Synonym Full Names
apolipoprotein L domain containing 1
NCBI Official Symbol
APOLD1
NCBI Official Synonym Symbols
VERGE
NCBI Protein Information
apolipoprotein L domain-containing protein 1; vascular early response gene protein
UniProt Protein Name
Apolipoprotein L domain-containing protein 1
Protein Family
UniProt Gene Name
APOLD1
UniProt Synonym Gene Names
VERGE
UniProt Entry Name
APLD1_HUMAN
NCBI Description
APOLD1 is an endothelial cell early response protein that may play a role in regulation of endothelial cell signaling and vascular function (Regard et al., 2004 [PubMed 15102925]).[supplied by OMIM, Dec 2008]
Uniprot Description
APOLD1: May be involved in angiogenesis. May play a role in activity-dependent changes of brain vasculature. May affect blood- brain permeability. Belongs to the apolipoprotein L family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Membrane protein, multi-pass; Lipid-binding
Chromosomal Location of Human Ortholog: 12p13.1
Cellular Component: plasma membrane; extracellular region; integral to membrane
Molecular Function: lipid binding
Biological Process: response to hypoxia; lipoprotein metabolic process; angiogenesis; cell differentiation; lipid transport; regulation of endothelial cell differentiation; endothelial cell activation
Research Articles on APOLD1
Similar Products
Product Notes
The Human APOLD1 apold1 (Catalog #AAA7232808) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7232808 ELISA Kit recognizes Human APOLD1. It is sometimes possible for the material contained within the vial of "Apolipoprotein L domain containing protein 1 (APOLD1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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