Typical Testing Data/Standard Curve
Human Anoctamin 5 (ANO5) ELISA Kit | ANO5 elisa kit
Human Anoctamin 5 (ANO5) ELISA Kit
Typical Testing Data/Standard Curve
Typical Testing Data/Standard Curve
Principle of the Assay||ANO5 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ANO5 antibody and an ANO5-HRP conjugate. The assay sample and buffer are incubated together with ANO5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ANO5 concentration since ANO5 from samples and ANO5-HRP conjugate compete for the anti-ANO5 antibody binding site. Since the number of sites is limited, as more sites are occupied by ANO5 from the sample, fewer sites are left to bind ANO5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ANO5 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the anoctamin family of transmembrane proteins. The encoded protein is likely a calcium activated chloride channel. Mutations in this gene have been associated with gnathodiaphyseal dysplasia. Alternatively spliced transcript variants have been described. [provided by RefSeq, Nov 2009]
Uniprot Description
ANO5: May act as a calcium-activated chloride channel. Defects in ANO5 are the cause of gnathodiaphyseal dysplasia (GDD); also known as osteogenesis imperfecta with unusual skeletal lesions or gnathodiaphyseal sclerosis. GDD is a rare skeletal syndrome characterized by bone fragility, sclerosis of tubular bones, and cemento-osseous lesions of the jawbone. Patients experience frequent bone fractures caused by trivial accidents in childhood; however the fractures heal normally without bone deformity. The jaw lesions replace the tooth-bearing segments of the maxilla and mandible with fibrous connective tissues, including various amounts of cementum-like calcified mass, sometimes causing facial deformities. Patients also have a propensity for jaw infection and often suffer from purulent osteomyelitis-like symptoms, such as swelling of and pus discharge from the gums, mobility of the teeth, insufficient healing after tooth extraction and exposure of the lesions into the oral cavity. Defects in ANO5 are the cause of limb-girdle muscular dystrophy type 2L (LGMD2L). It is an autosomal recessive degenerative myopathy characterized by proximal weakness, weakness of the hip and shoulder girdles and prominent asymmetrical quadriceps femoris and biceps brachii atrophy. Defects in ANO5 are the cause of miyoshi muscular dystrophy type 3 (MMD3). It is a late-onset muscular dystrophy characterized by distal muscle weakness of the lower limbs, calf muscle discomfort and weakness, quadriceps atrophy. Muscle weakness and atrophy may be asymmetric. Belongs to the anoctamin family.
Protein type: Membrane protein, integral; Membrane protein, multi-pass; Channel, chloride; Transporter; Transporter, ion channel
Chromosomal Location of Human Ortholog: 11p14.3
Cellular Component: endoplasmic reticulum membrane; integral to membrane; plasma membrane; intracellular; vesicle
Molecular Function: intracellular calcium activated chloride channel activity
Biological Process: chloride transport; transmembrane transport
Disease: Gnathodiaphyseal Dysplasia; Muscular Dystrophy, Limb-girdle, Type 2l; Miyoshi Muscular Dystrophy 3
Research Articles on ANO5
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Product Notes
The Human ANO5 ano5 (Catalog #AAA7203762) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7203762 ELISA Kit recognizes Human ANO5. It is sometimes possible for the material contained within the vial of "Anoctamin 5 (ANO5), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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