Human Annexin A9 (ANXA9) ELISA Kit | ANXA9 elisa kit

Human Annexin A9 (ANXA9) ELISA Kit

Cat. Num. AAA2021634

• Gene Names: ANXA9; ANX31
• Reactivity: Human
• Synonyms: Annexin A9 (ANXA9); Human Annexin A9 (ANXA9) ELISA Kit; ANX-A9; ANX31; Annexin-31; Pemphaxin; ANXA9 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Annexin A9 (ANXA9).; No significant cross-reactivity or interference between Annexin A9 (ANXA9) and analogues was observed.
• Sequence Length: 345

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.058ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Annexin A9 (ANXA9) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Annexin A9 (ANXA9) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ANXA9 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Annexin A9 (ANXA9). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Annexin A9 (ANXA9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Annexin A9 (ANXA9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Annexin A9 (ANXA9) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for ANXA9 elisa kit

Tumor immunity

NCBI and Uniprot Product Information

• NCBI GI #: 145864465
• NCBI GeneID: 8416
• NCBI Accession #: NP_003559.2
• NCBI GenBank Nucleotide #: NM_003568.2
• UniProt Accession #: O76027; Q5SZF1; Q6FI55; Q9BS00; Q9HBJ6
• Molecular Weight: 38,364 Da
• NCBI Official Full Name: annexin A9
• NCBI Official Synonym Full Names: annexin A9
• NCBI Official Symbol: ANXA9
• NCBI Official Synonym Symbols: ANX31
• NCBI Protein Information: annexin A9
• UniProt Protein Name: Annexin A9
• Protein Family: Annexin
• UniProt Gene Name: ANXA9
• UniProt Synonym Gene Names: ANX31
• UniProt Entry Name: ANXA9_HUMAN

NCBI Description

The annexins are a family of calcium-dependent phospholipid-binding proteins. Members of the annexin family contain 4 internal repeat domains, each of which includes a type II calcium-binding site. The calcium-binding sites are required for annexins to aggregate and cooperatively bind anionic phospholipids and extracellular matrix proteins. This gene encodes a divergent member of the annexin protein family in which all four homologous type II calcium-binding sites in the conserved tetrad core contain amino acid substitutions that ablate their function. However, structural analysis suggests that the conserved putative ion channel formed by the tetrad core is intact. [provided by RefSeq, Jul 2008]

Uniprot Description

ANXA9: a calcium/phospholipid-binding protein that may act as a low affinity receptor for acetylcholine. Annexins are a family of structurally related proteins whose common property is calcium-dependent binding to phospholipids. There are at least ten different annexins in mammalian species. Annexins do not contain signal peptides, yet some annexins (A1, A2 and A5) appear to be secreted in a physiologically regulated fashion.

Protein type: Lipid-binding; Calcium-binding

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: cell surface; cytosol

Molecular Function: protein binding; protein homodimerization activity; calcium-dependent phospholipid binding; phosphatidylserine binding; acetylcholine receptor activity; phospholipid binding; calcium ion binding

Biological Process: synaptic transmission; cell-cell adhesion