Human Annexin A7 (ANXA7) ELISA Kit | ANXA7 elisa kit

Human Annexin A7 (ANXA7) ELISA Kit

Cat. Num. AAA2098069

• Gene Names: ANXA7; SNX; ANX7; SYNEXIN
• Reactivity: Human
• Synonyms: Annexin A7 (ANXA7); Human Annexin A7 (ANXA7) ELISA Kit; ANX-A7; ANX7; SNX; Synexin; ANXA7 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Annexin A7 (ANXA7).; No significant cross-reactivity or interference between Annexin A7 (ANXA7) and analogues was observed.
• Sequence Length: 466

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.056ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Annexin A7 (ANXA7) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Annexin A7 (ANXA7) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ANXA7 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Annexin A7 (ANXA7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Annexin A7 (ANXA7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Annexin A7 (ANXA7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Annexin A7 (ANXA7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for ANXA7 elisa kit

Tumor immunity

NCBI and Uniprot Product Information

• NCBI GI #: 4502111
• NCBI GeneID: 310
• NCBI Accession #: NP_001147.1
• NCBI GenBank Nucleotide #: NM_001156.4
• UniProt Accession #: P20073; Q5F2H3; Q5T0M6; Q5T0M7
• Molecular Weight: 50,316 Da
• NCBI Official Full Name: annexin A7 isoform 1
• NCBI Official Synonym Full Names: annexin A7
• NCBI Official Symbol: ANXA7
• NCBI Official Synonym Symbols: SNX; ANX7; SYNEXIN
• NCBI Protein Information: annexin A7
• UniProt Protein Name: Annexin A7
• Protein Family: Annexin
• UniProt Gene Name: ANXA7
• UniProt Synonym Gene Names: ANX7; SNX

NCBI Description

Annexin VII is a member of the annexin family of calcium-dependent phospholipid binding proteins.The Annexin VII gene contains 14 exons and spans approximately 34 kb of DNA. An alternatively spliced cassette exon results in two mRNA transcripts of 2.0 and 2.4 kb which are predicted to generate two protein isoforms differing in their N-terminal domain. The alternative splicing event is tissue specific and the mRNA containing the cassette exon is prevalent in brain, heart and skeletal muscle. The transcripts also differ in their 3'-non coding regions by the use of two alternative poly(A) signals. Annexin VII encodes a protein with a molecular weight of approximately 51 kDa with a unique, highly hydrophobic N-terminal domain of 167 amino acids and a conserved C-terminal region of 299 amino acids. The latter domain is composed of alternating hydrophobic and hydrophilic segments. Structural analysis of the protein suggests that Annexin VII is a membrane binding protein with diverse properties, including voltage-sensitive calcium channel activity, ion selectivity and membrane fusion. [provided by RefSeq, Jul 2008]

Uniprot Description

Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis.