Human Angiomotin (AMOT) ELISA Kit | AMOT elisa kit

Human Angiomotin (AMOT) ELISA Kit

Cat. Num. AAA2704340

• Reactivity: Human
• Synonyms: Angiomotin (AMOT); Human Angiomotin (AMOT) ELISA Kit; AMOT elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Angiomotin (AMOT).; No significant cross-reactivity or interference between Angiomotin (AMOT) and analogues was observed.
• Sequence Length: 675

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.119ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Angiomotin (AMOT) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Angiomotin (AMOT) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AMOT elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Angiomotin (AMOT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Angiomotin (AMOT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Angiomotin (AMOT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Angiomotin (AMOT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 9887326
• NCBI GeneID: 154796
• UniProt Accession #: Q4VCS5; Q504X5; Q9HD27; Q9UPT1
• Molecular Weight: 72,540 Da
• NCBI Official Full Name: angiomotin
• NCBI Official Synonym Full Names: angiomotin
• NCBI Official Symbol: AMOT
• NCBI Protein Information: angiomotin
• UniProt Protein Name: Angiomotin
• Protein Family: Angiomotin
• UniProt Gene Name: AMOT
• UniProt Synonym Gene Names: KIAA1071

NCBI Description

This gene belongs to the motin family of angiostatin binding proteins characterized by conserved coiled-coil domains and C-terminal PDZ binding motifs. The encoded protein is expressed predominantly in endothelial cells of capillaries as well as larger vessels of the placenta where it may mediate the inhibitory effect of angiostatin on tube formation and the migration of endothelial cells toward growth factors during the formation of new blood vessels. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

Plays a central role in tight junction maintenance via the complex formed with ARHGAP17, which acts by regulating the uptake of polarity proteins at tight junctions. Appears to regulate endothelial cell migration and tube formation. May also play a role in the assembly of endothelial cell-cell junctions.