Human Angiomotin ELISA Kit | AMOT elisa kit

Human Angiomotin ELISA Kit

Cat. Num. AAA729758

• Reactivity: Human
• Synonyms: Angiomotin; Human Angiomotin ELISA Kit; AMOT elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 1.0-25ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This AMOT ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human AMOT. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AMOT elisa kit

Principle of the assay: AMOT ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AMOT antibody and an AMOT-HRP conjugate. The assay sample and buffer are incubated together with AMOT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AMOT concentration since AMOT from samples and AMOT-HRP conjugate compete for the anti-AMOT antibody binding site. Since the number of sites is limited, as more sites are occupied by AMOT from the sample, fewer sites are left to bind AMOT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AMOT concentration in each sample is interpolated from this standard curve.

Product Categories/Family for AMOT elisa kit

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 9887326
• NCBI GeneID: 154796
• Molecular Weight: 72,540 Da
• NCBI Official Full Name: angiomotin
• NCBI Official Synonym Full Names: angiomotin
• NCBI Official Symbol: AMOT
• NCBI Protein Information: angiomotin; angiomotin p80 isoform; angiomotin p130 isoform
• UniProt Protein Name: Angiomotin
• Protein Family: Angiomotin
• UniProt Gene Name: AMOT
• UniProt Synonym Gene Names: KIAA1071
• UniProt Entry Name: AMOT_HUMAN

NCBI Description

This gene belongs to the motin family of angiostatin binding proteins characterized by conserved coiled-coil domains and C-terminal PDZ binding motifs. The encoded protein is expressed predominantly in endothelial cells of capillaries as well as larger vessels of the placenta where it may mediate the inhibitory effect of angiostatin on tube formation and the migration of endothelial cells toward growth factors during the formation of new blood vessels. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

AMOT: Plays a central role in tight junction maintenance via the complex formed with ARHGAP17, which acts by regulating the uptake of polarity proteins at tight junctions. Appears to regulate endothelial cell migration and tube formation. May also play a role in the assembly of endothelial cell-cell junctions. Belongs to the angiomotin family. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: Xq23

Cellular Component: signalosome; ruffle; tight junction; cell surface; endocytic vesicle; lamellipodium; cytoplasm; integral to membrane; stress fiber; actin filament; cytosol; external side of plasma membrane

Molecular Function: angiostatin binding; protein binding; receptor activity

Biological Process: intercellular junction assembly; blood vessel endothelial cell migration; positive regulation of cell size; in utero embryonic development; positive regulation of blood vessel endothelial cell migration; gastrulation with mouth forming second; chemotaxis; regulation of cell migration; negative regulation of angiogenesis; regulation of small GTPase mediated signal transduction; positive regulation of embryonic development; positive regulation of stress fiber formation; negative regulation of vascular permeability; actin cytoskeleton organization and biogenesis; vasculogenesis; cell migration involved in gastrulation