Human Amiloride Sensitive Sodium Channel Subunit Alpha (SCNN1a) ELISA Kit | SCNN1a elisa kit

Human Amiloride Sensitive Sodium Channel Subunit Alpha (SCNN1a) ELISA Kit

Cat. Num. AAA2023473

• Reactivity: Human
• Synonyms: Amiloride Sensitive Sodium Channel Subunit Alpha (SCNN1a); Human Amiloride Sensitive Sodium Channel Subunit Alpha (SCNN1a) ELISA Kit; ENaCa; ENaCalpha; SCNEA; SCNN1; Sodium Channel; Nonvoltage-Gated 1; Alpha; Epithelial Na(+) channel subunit alpha; Nonvoltage-gated sodium channel 1 subunit alpha; SCNN1a elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Amiloride Sensitive Sodium Channel Subunit Alpha (SCNN1a).; No significant cross-reactivity or interference between Amiloride Sensitive Sodium Channel Subunit Alpha (SCNN1a) and analogues was observed.
• Sequence Length: 698

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.061ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Amiloride Sensitive Sodium Channel Subunit Alpha (SCNN1a) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Amiloride Sensitive Sodium Channel Subunit Alpha (SCNN1a) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SCNN1a elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Amiloride Sensitive Sodium Channel Subunit Alpha (SCNN1a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Amiloride Sensitive Sodium Channel Subunit Alpha (SCNN1a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Amiloride Sensitive Sodium Channel Subunit Alpha (SCNN1a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Amiloride Sensitive Sodium Channel Subunit Alpha (SCNN1a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for SCNN1a elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 13928742
• NCBI GeneID: 25122
• NCBI Accession #: NP_113736.1
• NCBI GenBank Nucleotide #: NM_031548.2
• UniProt Accession #: P37089; Q64593; Q9QUI4
• Molecular Weight: 78,888 Da
• NCBI Official Full Name: amiloride-sensitive sodium channel subunit alpha
• NCBI Official Synonym Full Names: sodium channel, non-voltage-gated 1 alpha subunit
• NCBI Official Symbol: Scnn1a
• NCBI Protein Information: amiloride-sensitive sodium channel subunit alpha
• UniProt Protein Name: Amiloride-sensitive sodium channel subunit alpha
• Protein Family: Amiloride-sensitive sodium channel
• UniProt Gene Name: Scnn1a
• UniProt Synonym Gene Names: Renac; Alpha-ENaC
• UniProt Entry Name: SCNNA_RAT

NCBI Description

acts as an epithelial sodium ion channel; regulates salt and fluid transport in the kidney [RGD, Feb 2006]

Uniprot Description

ENaC-alpha: Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception. Probable heterotrimer containing one alpha, one beta and one gamma subunit. A delta subunit can replace the alpha subunit. Interacts with the WW domains of NEDD4, NEDD4L, WWP1 and WWP2. By aldosterone. Highly expressed in kidney and lung. Detected at intermediate levels in pancreas and liver, and at low levels in heart and placenta. Isoform 1 and isoform 2 predominate in all tissues. Expression of isoform 3, isoform 4 and isoform 5 is very low or not detectable, except in lung and heart. Activated by WNK1, WNK2, WNK3 and WNK4. Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. SCNN1A subfamily. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Channel, sodium; Transporter, ion channel; Membrane protein, integral; Membrane protein, multi-pass

Cellular Component: cortical actin cytoskeleton; membrane; integral to plasma membrane; apical plasma membrane; integral to membrane; cytosol; external side of plasma membrane

Molecular Function: amiloride-sensitive sodium channel activity; WW domain binding; actin binding; sodium channel activity

Biological Process: regulation of body fluid levels; sensory perception of taste; regulation of blood pressure; sodium ion transport; sodium ion homeostasis; multicellular organismal water homeostasis