Human Amiloride-sensitive amine oxidase [copper-containing] (ABP1) ELISA Kit | ABP1 elisa kit

Human Amiloride-sensitive amine oxidase [copper-containing] (ABP1) ELISA Kit

Cat. Num. AAA286777

• Gene Names: AOC1; ABP; DAO; KAO; ABP1; DAO1
• Reactivity: Human
• Synonyms: Amiloride-sensitive amine oxidase [copper-containing] (ABP1); Human Amiloride-sensitive amine oxidase [copper-containing] (ABP1) ELISA Kit; ABP; AOC1; DAO; DAO1; KAO; amiloride binding protein 1; amiloride-binding protein; amiloride-binding protein-1; amiloride-sensitive amine oxidase; diamine oxidase; histaminase; kidney amine oxidase; ABP1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human ABP1. No significant cross-reactivity or interference between Human ABP1 and analogues was observed.
• Sequence Length: 751

• Assay Type: Quantitive Sandwich
• Samples: Serum, Plasma, Other Biological Fluids
• Detection Wavelength: 450 nm
• Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
• Inter-assay Precision (Precision between assays): Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
• CV (%): = SD/meanX100
• Intra-Assay: CV
• Inter-Assay: CV
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for ABP1 elisa kit

This assay employs a two-site sandwich ELISA to quantitate ABP1 in samples. An antibody specific for ABP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ABP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ABP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ABP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 73486661
• NCBI GeneID: 26
• NCBI Accession #: NP_001082.2
• NCBI GenBank Nucleotide #: NM_001091.3
• UniProt Accession #: P19801; Q16683; Q16684; Q56II4; Q6GU42; C9J690
• Molecular Weight: 87,239 Da
• NCBI Official Full Name: amiloride-sensitive amine oxidase
• NCBI Official Synonym Full Names: amine oxidase, copper containing 1
• NCBI Official Symbol: AOC1
• NCBI Official Synonym Symbols: ABP; DAO; KAO; ABP1; DAO1
• NCBI Protein Information: amiloride-sensitive amine oxidase [copper-containing]
• UniProt Protein Name: Amiloride-sensitive amine oxidase [copper-containing]
• Protein Family: ARS-binding protein
• UniProt Gene Name: AOC1
• UniProt Synonym Gene Names: ABP1; DAO1; DAO; Diamine oxidase; KAO

NCBI Description

This gene encodes a metal-binding membrane glycoprotein that oxidatively deaminates putrescine, histamine, and related compounds. The encoded protein is inhibited by amiloride, a diuretic that acts by closing epithelial sodium ion channels. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2013]

Uniprot Description

Catalyzes the degradation of compounds such as putrescine, histamine, spermine, and spermidine, substances involved in allergic and immune responses, cell proliferation, tissue differentiation, tumor formation, and possibly apoptosis. Placental DAO is thought to play a role in the regulation of the female reproductive function.MiscellaneousInhibited by amiloride in a competitive manner.