Human Alpha-2-Macroglobulin ELISA Kit | A2M elisa kit

Human Alpha-2-Macroglobulin ELISA Kit

Cat. Num. AAA135938

• Gene Names: A2M; A2MD; CPAMD5; FWP007; S863-7
• Reactivity: Human
• Synonyms: Alpha-2-Macroglobulin; Human Alpha-2-Macroglobulin ELISA Kit; A2M elisa kit

• Reactivity: Human
• Form/Format: Complete kit
• Sequence Length: 1474

• Source Info: Multiple
• Target Info: Alpha-2-macroglobulin
• Preparation and Storage: Recommended Storage: 4 degree C; Minimum Shelf Life: 6 months from manufacture, see label for expiration date; Shipping Condition: Cold pack

Related Product Information for A2M elisa kit

Alpha-2-macroglobulin (A2M) is a circulating 720 kDa homotetramer expressed in the liver which captures a wide range of plasma proteinases including plasmin and thrombin. Each monomer contains an internal thiol ester, a transglutaminase reactive site, zinc and receptor binding sites, and a bait region which when cleaved induces a conformational change trapping the proteinase. Serum A2M levels are decreased in acute pancreatitis and increased in chronic liver disease and nephrotic syndrome. The sensitive quantitative measurement of total human alpha-2-macroglobulin in samples is easily performed with this 96 well strip format ELISA kit. The normal human concentration of macroglobulin is 1.2 mg/ml in plasma. The assay measures total macroglobulin in the 1-250 ng/ml range. Samples giving human macroglobulin levels above 250 ng/ml should be diluted in blocking buffer before use. A 1:100,000 dilution for plasma is suggested for best results. Human macroglobulin will bind to the capture antibody coated onto a micro titer plate. After appropriate washing steps, anti human antiplasmin primary antibody binds to the antiplasmin. Excess antibody is washed away, and bound antibody is reacted with peroxidase conjugated secondary antibody. TMB substrate is used for color development at 450 nm. Color development is proportional to the concentration of macroglobulin in the samples. A standard calibration curve is prepared using dilutions of purified macroglobulin and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

Product Categories/Family for A2M elisa kit

HUMAN PLASMA PROTEINS

NCBI and Uniprot Product Information

• NCBI GI #: 66932947
• NCBI GeneID: 2
• NCBI Accession #: NP_000005.2
• NCBI GenBank Nucleotide #: NM_000014.4
• UniProt Accession #: P01023; Q13677; Q59F47; Q5QTS0; Q68DN2; Q6PIY3; Q6PN97
• Molecular Weight: 163,291 Da
• NCBI Official Full Name: alpha-2-macroglobulin
• NCBI Official Synonym Full Names: alpha-2-macroglobulin
• NCBI Official Symbol: A2M
• NCBI Official Synonym Symbols: A2MD; CPAMD5; FWP007; S863-7
• NCBI Protein Information: alpha-2-macroglobulin; alpha-2-M; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5
• UniProt Protein Name: Alpha-2-macroglobulin
• Protein Family: Alpha-2-macroglobulin
• UniProt Gene Name: A2M
• UniProt Synonym Gene Names: CPAMD5; Alpha-2-M
• UniProt Entry Name: A2MG_HUMAN

NCBI Description

Alpha-2-macroglobulin is a protease inhibitor and cytokine transporter. It inhibits many proteases, including trypsin, thrombin and collagenase. A2M is implicated in Alzheimer disease (AD) due to its ability to mediate the clearance and degradation of A-beta, the major component of beta-amyloid deposits. [provided by RefSeq, Jul 2008]

Uniprot Description

A2M: Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase. Belongs to the protease inhibitor I39 (alpha-2- macroglobulin) family.

Protein type: Secreted; Secreted, signal peptide; Inhibitor

Chromosomal Location of Human Ortholog: 12p13.31

Cellular Component: extracellular region; cytosol

Molecular Function: serine-type endopeptidase inhibitor activity; protein binding; enzyme binding; protease binding; growth factor binding; interleukin-8 binding; interleukin-1 binding; calcium-dependent protein binding; tumor necrosis factor binding; receptor binding

Biological Process: negative regulation of complement activation, lectin pathway; platelet activation; extracellular matrix disassembly; extracellular matrix organization and biogenesis; regulation of small GTPase mediated signal transduction; platelet degranulation; small GTPase mediated signal transduction; stem cell differentiation; blood coagulation; blood coagulation, intrinsic pathway

Disease: Alzheimer Disease; Alpha-2-macroglobulin Deficiency