Typical Testing Data/Standard Curve (for reference only)
Human Alpha 1, 6 mannosylglycoprotein 6 beta N acetylglucosaminyltransferase A (MGAT5) ELISA Kit | MGAT5 elisa kit
Human Alpha 1, 6 mannosylglycoprotein 6 beta N acetylglucosaminyltransferase A (MGAT5) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: MGAT5 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-MGAT5 antibody and an MGAT5-HRP conjugate. The assay sample and buffer are incubated together with MGAT5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MGAT5 concentration since MGAT5 from samples and MGAT5-HRP conjugate compete for the anti-MGAT5 antibody binding site. Since the number of sites is limited, as more sites are occupied by MGAT5 from the sample, fewer sites are left to bind MGAT5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MGAT5 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the glycosyltransferase family. It catalyzes the addition of beta-1,6-N-acetylglucosamine to the alpha-linked mannose of biantennary N-linked oligosaccharides present on the newly synthesized glycoproteins. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides. Alterations of the oligosaccharides on cell surface glycoproteins cause significant changes in the adhesive or migratory behavior of a cell. Increase in the activity of this enzyme has been correlated with the progression of invasive malignancies. [provided by RefSeq, Oct 2011]
Uniprot Description
MGAT5: Catalyzes the addition of N-acetylglucosamine in beta 1- 6 linkage to the alpha-linked mannose of biantennary N-linked oligosaccharides. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides. Belongs to the glycosyltransferase 18 family.
Protein type: Membrane protein, integral; Glycan Metabolism - N-glycan biosynthesis; EC 2.4.1.155; Transferase
Chromosomal Location of Human Ortholog: 2q21.3
Cellular Component: Golgi membrane; integral to membrane
Molecular Function: alpha-1,6-mannosyl-glycoprotein 6-beta-N-acetylglucosaminyltransferase activity
Biological Process: cellular protein metabolic process; protein amino acid N-linked glycosylation via asparagine; post-translational protein modification
Research Articles on MGAT5
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Product Notes
The Human MGAT5 mgat5 (Catalog #AAA7209837) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7209837 ELISA Kit recognizes Human MGAT5. It is sometimes possible for the material contained within the vial of "Alpha 1, 6 mannosylglycoprotein 6 beta N acetylglucosaminyltransferase A (MGAT5), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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