Human Aldo keto reductase family 1 member C4 (AKR1C4) ELISA Kit | AKR1C4 elisa kit

Human Aldo keto reductase family 1 member C4 (AKR1C4) ELISA Kit

Cat. Num. AAA7212263

• Gene Names: AKR1C4; C11; CDR; DD4; CHDR; DD-4; HAKRA; 3-alpha-HSD
• Reactivity: Human
• Synonyms: Aldo keto reductase family 1 member C4 (AKR1C4); Human Aldo keto reductase family 1 member C4 (AKR1C4) ELISA Kit; AKR1C4 elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 250-5000pg/mL
• Sensitivity: 1.0pg/mL
• Intended Uses: This AKR1C4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human AKR1C4. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AKR1C4 elisa kit

Priciple of the assay: AKR1C4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AKR1C4 antibody and an AKR1C4-HRP conjugate. The assay sample and buffer are incubated together with AKR1C4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AKR1C4 concentration since AKR1C4 from samples and AKR1C4-HRP conjugate compete for the anti-AKR1C4 antibody binding site. Since the number of sites is limited, as more sites are occupied by AKR1C4 from the sample, fewer sites are left to bind AKR1C4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AKR1C4 concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 325652083
• NCBI GeneID: 1109
• NCBI Accession #: NP_001809.3
• NCBI GenBank Nucleotide #: NM_001818.3
• Molecular Weight: 37,067 Da
• NCBI Official Full Name: aldo-keto reductase family 1 member C4
• NCBI Official Synonym Full Names: aldo-keto reductase family 1, member C4
• NCBI Official Symbol: AKR1C4
• NCBI Official Synonym Symbols: C11; CDR; DD4; CHDR; DD-4; HAKRA; 3-alpha-HSD
• NCBI Protein Information: aldo-keto reductase family 1 member C4; 3-alpha-HSD1; dihydrodiol dehydrogenase isozyme DD4; type I 3-alpha-hydroxysteroid dehydrogenase; chlordecone reductase; 3-alpha hydroxysteroid dehydrogenase, type I; dihydrodiol dehydrogenase 4
• UniProt Protein Name: Aldo-keto reductase family 1 member C4
• Protein Family: Aldo-keto reductase family
• UniProt Gene Name: AKR1C4
• UniProt Synonym Gene Names: CHDR; CDR; DD-4; DD4
• UniProt Entry Name: AK1C4_HUMAN

NCBI Description

This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the bioreduction of chlordecone, a toxic organochlorine pesticide, to chlordecone alcohol in liver. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. [provided by RefSeq, Jul 2008]

Uniprot Description

AKR1C4: Catalyzes the transformation of the potent androgen dihydrotestosterone (DHT) into the less active form, 5-alpha- androstan-3-alpha,17-beta-diol (3-alpha-diol). Also has some 20- alpha-hydroxysteroid dehydrogenase activity. The biotransformation of the pesticide chlordecone (kepone) to its corresponding alcohol leads to increased biliary excretion of the pesticide and concomitant reduction of its neurotoxicity since bile is the major excretory route. Defects in AKR1C4 are a cause of 46,XY sex reversal type 8 (SRXY8). A disorder of sex development. Affected individuals have a 46,XY karyotype but present as phenotypically normal females. AKR1C4 mutations may act as modifier of disease severity in SRXY8 patients. A splicing mutation resulting in loss of exon 2 has been found in affected individuals also carrying mutation Val-79 in AKR1C2 (PubMed:21802064). Belongs to the aldo/keto reductase family.

Protein type: EC 1.1.1.357; Lipid Metabolism - C21-steroid hormone; Lipid Metabolism - androgen and estrogen; EC 1.1.1.225; Oxidoreductase; Lipid Metabolism - primary bile acid biosynthesis; Xenobiotic Metabolism - metabolism by cytochrome P450

Chromosomal Location of Human Ortholog: 10p15.1

Cellular Component: cytoplasm; cytosol

Molecular Function: oxidoreductase activity, acting on NADH or NADPH, quinone or similar compound as acceptor; electron carrier activity; aldo-keto reductase activity; chlordecone reductase activity; bile acid transmembrane transporter activity; 3(or 17)-alpha-hydroxysteroid dehydrogenase activity; retinal dehydrogenase activity

Biological Process: bile acid and bile salt transport; steroid metabolic process; bile acid biosynthetic process; phototransduction, visible light; bile acid metabolic process; androgen metabolic process; retinoid metabolic process

Disease: 46,xy Sex Reversal 8