Typical Testing Data/Standard Curve (for reference only)
Human Aldo-keto reductase family 1 member B10 (AKR1B10) ELISA Kit | AKR1B10 elisa kit
Human Aldo-keto reductase family 1 member B10 (AKR1B10) ELISA Kit
Gene Names
AKR1B10; HIS; HSI; ARL1; ARL-1; ALDRLn; AKR1B11; AKR1B12
Reactivity
Human
Synonyms
Aldo-keto reductase family 1 member B10 (AKR1B10); Human Aldo-keto reductase family 1 member B10 (AKR1B10) ELISA Kit; AKR1B10 elisa kit
Reactivity
Human
Sequence Length
316
Samples
Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
Assay Type
Quantitative Sandwich
Detection Range
0.05ng/ml - 10ng/ml
Sensitivity
0.021ng/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for AKR1B10 elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of human Aldo-keto Reductase Family 1 Member B10 (also known as AKR1B10) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human AKR1B10 antibody. AKR1B10 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human AKR1B10 Antibody is added and binds to AKR1B10 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated AKR1B10 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human AKR1B10. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human AKR1B10 antibody. AKR1B10 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human AKR1B10 Antibody is added and binds to AKR1B10 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated AKR1B10 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human AKR1B10. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
36,020 Da
NCBI Official Full Name
aldo-keto reductase family 1 member B10
NCBI Official Synonym Full Names
aldo-keto reductase family 1 member B10
NCBI Official Symbol
AKR1B10
NCBI Official Synonym Symbols
HIS; HSI; ARL1; ARL-1; ALDRLn; AKR1B11; AKR1B12
NCBI Protein Information
aldo-keto reductase family 1 member B10
UniProt Protein Name
Aldo-keto reductase family 1 member B10
Protein Family
UniProt Gene Name
AKR1B10
UniProt Synonym Gene Names
AKR1B11; ARP; hARP; SI reductase
NCBI Description
This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member can efficiently reduce aliphatic and aromatic aldehydes, and it is less active on hexoses. It is highly expressed in adrenal gland, small intestine, and colon, and may play an important role in liver carcinogenesis. [provided by RefSeq, Jul 2008]
Uniprot Description
Acts as all-trans-retinaldehyde reductase. Can efficiently reduce aliphatic and aromatic aldehydes, and is less active on hexoses (in vitro). May be responsible for detoxification of reactive aldehydes in the digested food before the nutrients are passed on to other organs.
Research Articles on AKR1B10
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Product Notes
The Human AKR1B10 akr1b10 (Catalog #AAA166747) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA166747 ELISA Kit recognizes Human AKR1B10. It is sometimes possible for the material contained within the vial of "Aldo-keto reductase family 1 member B10 (AKR1B10), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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